ArticleMicrobiologyOpen2021
Development and clinical validation of a droplet digital PCR assay for detecting Acinetobacter baumannii and Klebsiella pneumoniae in patients with suspected bloodstream infections.
Article in MicrobiologyOpen, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Microbial architects of cigar fermentation: a critical review of beneficial roles in quality enhancement and detrimental potential for mould spoilage.Archives of microbiology · 2026Review
- Rapid Detection of Pathogens and Resistance Genes in Positive Blood Cultures Using BioFirePolish journal of microbiology · 2026Article
- cfGWAS reveal genetic basis of cell-free DNA end motifs.Nature communications · 2026Article
- Diagnostic Performance of Droplet Digital PCR for Detecting Pathogens and Resistance Genes in Patients with Suspected Bloodstream Infections.Infection and drug resistance · 2026Article
- Optimizing Clinical Indicators in Hematologic Malignancies and Sepsis Using ddPCR: A Retrospective Study.Infectious diseases and therapy · 2025Article
- Phenotypic and molecular phylogeny ofJournal of advanced veterinary and animal research · 2025Article
- Leveraging innovative diagnostics as a tool to contain superbugs.Antonie van Leeuwenhoek · 2025Review
- Comparative analysis between digital PCR and blood culture for blood pathogen detection.Frontiers in medicine · 2025Article
- Establishment and validation of a dual qPCR method for the detection of carbapenem-resistantFrontiers in cellular and infection microbiology · 2025Article
- Droplet Digital PCR forAntibiotics (Basel, Switzerland) · 2024Article
- Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings.Diagnostics (Basel, Switzerland) · 2024Review
- Development of a duplex droplet digital PCR assay for the detection ofMicrobiology spectrum · 2024Article
- A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR.Heliyon · 2024Article
- Analytical and clinical validation of multiplex droplet digital PCR assay for detecting pathogenic fungal infection in lungs.Mycology · 2024Article
- Reliability of Droplet Digital PCR Alone and in Combination with Interleukin-6 and Procalcitonin for Prognosis of Bloodstream Infection.Infection and drug resistance · 2024Article
- Development and clinical validation of a dual ddPCR assay for detecting carbapenem-resistantFrontiers in microbiology · 2024Article
- Clinical evaluation of a multiplex droplet digital PCR for diagnosing suspected bloodstream infections: a prospective study.Frontiers in cellular and infection microbiology · 2024Article
- Clinical evaluation of droplet digital PCR in the early identification of suspected sepsis patients in the emergency department: a prospective observational study.Frontiers in cellular and infection microbiology · 2024Observational
- Relationship between clinical features and droplet digital PCR copy number in non-HIV patients with pneumocystis pneumonia.BMC infectious diseases · 2023Article
- Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR.Microbiology spectrum · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relatively long turnaround time and low sensitivity of traditional blood culture-based diagnosis may delay effective antibiotic therapy for patients with bloodstream infections (BSIs). A rapid and sensitive pathogen detection method is urgently required to reduce the morbidity and mortality associated with BSIs. Acinetobacter baumannii and Klebsiella pneumoniae are two major microorganisms that cause BSIs. Here we report a novel droplet digital polymerase chain reaction (ddPCR) assay that can detect A. baumannii and K. pneumoniae in blood samples within 4 h, with a specificity of 100% for each strain and a limit of detection at 0.93 copies/μl for A. baumannii and 0.27 copies/μl for K. pneumoniae. Clinical validation of 170 patients with suspected BSIs showed that compared to blood cultures that detected four (2.4%) A. baumannii cases and seven (4.1%) K. pneumoniae cases, ddPCR detected 23 (13.5%) A. baumannii cases, 26 (15.3%) K. pneumoniae cases, and four (2.4%) co-infection cases, including the 11 cases detected via blood culture. In addition, patients who tested positive via ddPCR alone (n = 42) had significantly lower serum concentrations of procalcitonin and lactate, SOFA and APACHE II scores, and 28-day mortality than those reported positive via both blood culture and ddPCR (n = 11), suggesting that patients with less severe symptoms can potentially benefit from ddPCR-based diagnosis. In conclusion, our study suggests that ddPCR represents a sensitive and rapid method for identifying causal pathogens in blood samples and guiding treatment decisions in the early stages of BSIs.
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