ArticlePlants (Basel, Switzerland)2021
Optimization of Protoplast Isolation from Leaf Mesophylls of Chinese Cabbage (
Article in Plants (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Systematic Evaluation of Mesophyll Protoplast Isolation and PEG-Mediated Transient Expression inPlants (Basel, Switzerland) · 2026Article
- An Efficient Regeneration and Transient Transformation System for the Desert HalophytePlants (Basel, Switzerland) · 2026Article
- ExploitingPlants (Basel, Switzerland) · 2024Review
- Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023Article
- Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruitFrontiers in plant science · 2023Article
- Optimization of cabbage (Frontiers in plant science · 2023Article
- Optimization of an Efficient Protoplast Transformation System for Transient Expression Analysis Using Leaves ofPlants (Basel, Switzerland) · 2022Article
- Optimization of Protoplast Isolation and Transformation for a Pilot Study of Genome Editing in Peanut by Targeting the Allergen GeneInternational journal of molecular sciences · 2022Article
- A high-efficiency PEG-CaFrontiers in plant science · 2022Article
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Authors and funding
6 authors.
Funding
Abstract
Chinese cabbage is an important dietary source of numerous phytochemicals, including glucosinolates and anthocyanins. The selection and development of elite Chinese cabbage cultivars with favorable traits is hindered by a long breeding cycle, a complex genome structure, and the lack of an efficient plant transformation protocol. Thus, a protoplast transfection-based transformation method may be useful for cell-based breeding and functional studies involving Chinese cabbage plants. In this study, we established an effective method for isolating Chinese cabbage protoplasts, which were then transfected with the pCAMBIA1303 binary vector according to an optimized PEG-based method. More specifically, protoplasts were isolated following a 4 h incubation in a solution comprising 1.5% (
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