Evidence map›Paper›PMID 34960682›Full record

ArticleViruses2021

Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments.

Martina Pavletić, Marija Mazor, Mate Lerga, Tatjana Mileta, Jelena Železnjak, Tina Ružić, Sanda Ravlić, Dora Palčevski, Jelena Kirinčić, Silvestar Mežnarić and 5 more

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.1field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Secreted small RNAs ofbioRxiv : the preprint server for biology · 2025
    Article
  4. Outbreak of hantavirus disease caused by Puumala virus, Croatia, 2021.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Martina PavletićEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Marija MazorCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.ORCID 0000-0002-3670-6334
Mate LergaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Tatjana MiletaEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Jelena ŽeleznjakCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
Tina RužićCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.ORCID 0000-0002-1995-9620
Sanda RavlićCentre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, 10000 Zagreb, Croatia.
Dora PalčevskiEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Jelena KirinčićEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Silvestar MežnarićEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Ana MiškovićEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Maja MaterljanEmergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Alan ŠustićDepartment of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
Berislav LisnićCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
Vanda Juranić LisnićCenter for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.ORCID 0000-0002-7182-8927
University of Rijeka · HRUniversity of Zagreb · HR

Funding

Croatian Science Foundation IP-CORONA-04-2053European Regional Development Fund KK.01.1.1.01.0006
6 · The paper itself

Abstract

During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.

Indexed as

Emergency Service, HospitalPoint-of-Care TestingCOVID-19COVID-19 Nucleic Acid TestingCroatiaHumansReproducibility of ResultsRNA, ViralSARS-CoV-2Sensitivity and SpecificityRNA, ViralCOVID-19direct qPCRdqPCRemergency departmentmolecular diagnosticspoint-of-care PCRqPCRSARS-CoV-2

Identifiers

PMID34960682
PMCPMC8707628
OpenAlexW3216298146

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.