Evidence map›Paper›PMID 34960658›Full record

ArticleViruses2021

HIV-1 Packaging Visualised by In-Gel SHAPE.

Aaron R D'Souza, Dhivya Jayaraman, Ziqi Long, Jingwei Zeng, Liam J Prestwood, Charlene Chan, Dennis Kappei, Andrew M L Lever, Julia C Kenyon

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Aaron R D'SouzaDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Dhivya JayaramanDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Ziqi LongDepartment of Medicine, University of Cambridge, Level 5 Addenbrookes Hospital, Cambridge CB2 0QQ, UK.ORCID 0000-0002-4666-9936
Jingwei ZengDepartment of Medicine, University of Cambridge, Level 5 Addenbrookes Hospital, Cambridge CB2 0QQ, UK.ORCID 0000-0002-9802-6019
Liam J PrestwoodDepartment of Medicine, University of Cambridge, Level 5 Addenbrookes Hospital, Cambridge CB2 0QQ, UK.
Charlene ChanCancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.
Dennis KappeiCancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.ORCID 0000-0002-3582-2253
Andrew M L LeverDepartment of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Julia C KenyonCancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.ORCID 0000-0001-6055-7052

Funding

Medical Research Council MR/N022939/1
6 · The paper itself

Abstract

HIV-1 packages two copies of its gRNA into virions via an interaction with the viral structural protein Gag. Both copies and their native RNA structure are essential for virion infectivity. The precise stepwise nature of the packaging process has not been resolved. This is largely due to a prior lack of structural techniques that follow RNA structural changes within an RNA-protein complex. Here, we apply the in-gel SHAPE (selective 2'OH acylation analysed by primer extension) technique to study the initiation of HIV-1 packaging, examining the interaction between the packaging signal RNA and the Gag polyprotein, and compare it with that of the NC domain of Gag alone. Our results imply interactions between Gag and monomeric packaging signal RNA in switching the RNA conformation into a dimerisation-competent structure, and show that the Gag-dimer complex then continues to stabilise. These data provide a novel insight into how HIV-1 regulates the translation and packaging of its genome.

Indexed as

Virus Assemblygag Gene Products, Human Immunodeficiency VirusGenome, ViralHIV-1HIV InfectionsHumansNucleic Acid ConformationRNA, Viralgag Gene Products, Human Immunodeficiency VirusRNA, ViraldimerisationGagHIV-1in-gel SHAPENCpackagingRNA structure

Identifiers

PMID34960658
PMCPMC8707378

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.