ArticleNutrients2021
The Viable Microbiome of Human Milk Differs from the Metataxonomic Profile.
Article in Nutrients, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Human milk microbiome as a modulator of the early-life gut-brain axis: mechanisms and translational opportunities for neurodevelopment.Journal of translational medicine · 2026Review
- The Impact of Cold Storage and Seasonality on Raw Cow Milk Microbiota, Assessed by Conventional and Viability PCR.Animals : an open access journal from MDPI · 2026Article
- Human milk microbiota: origins, determinants, and roles in maternal-infant microbial transmission and infant microbiome assembly.Frontiers in microbiology · 2026Review
- Seeding and feeding: nutrition and birth-associated exposures shape gut microbiome assembly in breastfed infants.Gut microbes · 2025Article
- Article
- Viable bacterial communities in freshly pumped human milk and their changes during cold storage conditions.International breastfeeding journal · 2025Observational
- Propidium Monoazide is Unreliable for Quantitative Live-Dead Molecular Assays.Analytical chemistry · 2025Article
- The impact of breastfeeding on the preterm infant's microbiome and metabolome: a pilot study.Pediatric research · 2025Observational
- Milk microbiome transplantation: recolonizing donor milk with mother's own milk microbiota.Applied microbiology and biotechnology · 2024Article
- Illumina Sequencing in Conjunction with Propidium Monoazide to Identify Live Bacteria After Antiseptic Treatment in a Complex Oral Biofilm: A Study Using an Ex Vivo Supragingival Biofilm Model.Antibiotics (Basel, Switzerland) · 2024Article
- Residents or Tourists: Is the Lactating Mammary Gland Colonized by Residential Microbiota?Microorganisms · 2024Review
- Microbiome: Mammalian milk microbiomes: sources of diversity, potential functions, and future research directions.Reproduction & fertility · 2024Review
- Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniques.ISME communications · 2023Article
- Early-life interactions between the microbiota and immune system: impact on immune system development and atopic disease.Nature reviews. Immunology · 2023Review
- Evidence for human milk as a biological system and recommendations for study design-a report from "Breastmilk Ecology: Genesis of Infant Nutrition (BEGIN)" Working Group 4.The American journal of clinical nutrition · 2023Article
- Improvement and Validation of a Genomic DNA Extraction Method for Human Breastmilk.Methods and protocols · 2023Article
- Insufficient Evidence of a Breastmilk Microbiota at Six-Weeks Postpartum: A Pilot Study.Nutrients · 2023Article
- Metagenomic assessment of the bacterial breastfeeding microbiome in mature milk across lactation.Frontiers in pediatrics · 2023Article
- The Application of Metagenomics to Study Microbial Communities and Develop Desirable Traits in Fermented Foods.Foods (Basel, Switzerland) · 2022Review
- Gut microbiome and breast-feeding: Implications for early immune development.The Journal of allergy and clinical immunology · 2022Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Bacteria in human milk contribute to the establishment of the infant gut microbiome. As such, numerous studies have characterized the human milk microbiome using DNA sequencing technologies, particularly 16S rRNA gene sequencing. However, such methods are not able to differentiate between DNA from viable and non-viable bacteria. The extent to which bacterial DNA detected in human milk represents living, biologically active cells is therefore unclear. Here, we characterized both the viable bacterial content and the total bacterial DNA content (derived from viable and non-viable cells) of fresh human milk (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.