Evidence map›Paper›PMID 34958187›Full record

ArticleJournal of structural biology: X

Structural and biochemical characterization of the pleckstrin homology domain of the RhoGEF P-Rex2 and its regulation by PIP

Jennifer N Cash, Prateek V Sharma, John J G Tesmer

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology: X. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Inositol-Exchange Activity in Human Primordial Placenta.International journal of molecular sciences · 2024
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Jennifer N CashDepartment of Pharmacology, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2216, USA.
Prateek V SharmaDepartment of Pharmacology, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2216, USA.
John J G TesmerDepartment of Biological Sciences and of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47904, USA.
University of Michigan–Ann Arbor · USPurdue University West Lafayette · US

Funding

Structure, function, and inhibition of G protein-coupled receptor kinasesR01HL071818 · NHLBI · UNIVERSITY OF TEXAS AUSTIN · PI TESMER, JOHN · 2004 to 2023
$7.1M
Structure and Function of the LPLA2/LCAT Acyltransferase FamilyR01HL122416 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TESMER, JOHN · 2015 to 2018
$1.9M
GPCR - Linked RhoGEFs in Tumor Growth and MetastasisR01CA221289 · NCI · PURDUE UNIVERSITY · PI TESMER, JOHN · 2018 to 2022
$1.9M
NCI NIH HHS R01 CA221289NHLBI NIH HHS R01 HL071818NHLBI NIH HHS R01 HL122416
6 · The paper itself

Abstract

P-Rex family Rho guanine-nucleotide exchange factors are important regulators of cell motility through their activation of a subset of small GTPases. Both P-Rex1 and P-Rex2 have also been implicated in the progression of certain cancers, including breast cancer and melanoma. Although these molecules display a high level of homology, differences exist in tissue distribution, physiological function, and regulation at the molecular level. Here, we sought to compare the P-Rex2 pleckstrin homology (PH) domain structure and ability to interact with PIP

Indexed as

DEP, dishevelled, Egl-10, and pleckstrinDH, Dbl homologyDSF, differential scanning fluorimetryDTT, dithiothreitolEDTA, ethylenediaminetetraaceticGβγ, G protein β and γ subunitsIns(1,3,4,5)P4, inositol-1,3,4,5-tetrakisphosphateIP4P, inositol polyphosphate 4-phosphataseMBP, maltose binding proteinPDZ, post-synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 proteinPhosphatidylinositol 3,4,5-trisphosphatePH, pleckstrin homologyPIP3, phosphatidylinositol 3,4,5-trisphosphatePleckstrin homology domainPMSF, phenylmethylsulfonyl fluorideP-RexP-Rex, phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchangerPTEN, phosphatase and tensin homologRhoGEF, Rho guanine-nucleotide exchange factorRho guanine nucleotide exchange factor

Identifiers

PMID34958187
PMCPMC7337056
OpenAlexW2903797610

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.