ArticleJournal of animal science and technology2021
Decoding the intestinal microbiota repertoire of sow and weaned pigs using culturomic and metagenomic approaches.
Article in Journal of animal science and technology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Copper is an intestinal habitat filter affecting the gut microbiota interactions with Salmonella Typhimurium.Microbiome · 2026Article
- Dynamic Interactions between the Gut Microbiome, Health, and Metabolic Disorders in Preterm Infants.Journal of microbiology and biotechnology · 2025Review
- Intestinal microbiota dynamics in piglets: the interplay with swine enteric coronavirus infections and implications for disease control.Animal microbiome · 2025Review
- Biodiversity and identification of spore-forming bacteria in raw milk from Korean dairy farm environment using the culturomics approach.Journal of animal science and technology · 2025Article
- Structure-dependent degradation of milk oligosaccharides by newly isolated intestinal commensal bacterial strains from suckling piglets and rabbits.BMC microbiology · 2025Article
- Integrative Analysis of Probiotic-Mediated Remodeling in Canine Gut Microbiota and Metabolites Using a Fermenter for an Intestinal Microbiota Model.Food science of animal resources · 2024Article
- Dispersion of antimicrobial resistant bacteria in pig farms and in the surrounding environment.Animal microbiome · 2024Article
- Bisphenol A exposure affects specific gut taxa and drives microbiota dynamics in childhood obesity.mSystems · 2024Article
- Dietary supplementation with probiotics promotes weight loss by reshaping the gut microbiome and energy metabolism in obese dogs.Microbiology spectrum · 2024Article
- Isolation and Pathogenicity of a Chinese Porcine Astrovirus Type 5 Strain HNPDS-01 and Its Influence on Cecum Microbiota in Piglets.Transboundary and emerging diseases · 2024Article
- Culturomic-, metagenomic-, and transcriptomic-based characterization of commensal lactic acid bacteria isolated from domestic dogs using Caenorhabditis elegans as a model for aging.Journal of animal science · 2022Article
- The Ingestion of Dietary Prebiotic Alternatives during Lactation Promotes Intestinal Health by Modulation of Gut Microbiota.Journal of microbiology and biotechnology · 2022Article
- Molecular characterization of gut microbiome in weaning pigs supplemented with multi-strain probiotics using metagenomic, culturomic, and metabolomic approaches.Animal microbiome · 2022Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To elucidate the role and mechanism of microbes, we combined culture-dependent and culture-independent approaches to investigate differences in gut bacterial composition between sows and weaned pigs. Under anaerobic conditions, several nonselective and selective media were used for isolation from fecal samples. All isolated bacteria were identified and classified through 16S rRNA sequencing, and the microbiota composition of the fecal samples was analyzed by metagenomics using next generation sequencing (NGS) technology. A total of 278 and 149 colonies were acquired from the sow and weaned pig fecal samples, respectively. Culturomics analysis revealed that diverse bacterial genus and species belonged to Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes were isolated from sow and weaned pigs. When comparing culture-dependent and culture-independent analyses, 191 bacterial species and 2 archaeal bacterial species were detected through culture-independent analysis, and a total of 23 bacteria were isolated through a culture-dependent approach, of which 65% were not detected by metagenomics. In conclusion, culturomics and metagenomics should be properly combined to fully understand the intestinal microbiota, and livestock-derived microbial resources should be informed by culturomic approaches to understand and utilize the mechanism of host-microbe interactions.
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