Evidence map›Paper›PMID 34957215›Full record

ArticleFrontiers in molecular biosciences2021

Determination of Histidine Protonation States in Proteins by Fast Magic Angle Spinning NMR.

Roman Zadorozhnyi, Sucharita Sarkar, Caitlin M Quinn, Kaneil K Zadrozny, Barbie K Ganser-Pornillos, Owen Pornillos, Angela M Gronenborn, Tatyana Polenova

Abstract read
In one paragraph

Article in Frontiers in molecular biosciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. In Situ Light-driven pH Modulation for NMR Studies.Angewandte Chemie (International ed. in English) · 2025
    Article
  2. Article
  3. Simultaneous recoupling of chemical shift tensors of two nuclei by R-symmetry sequences.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2023
    Article
  4. A Chemical Biology Primer for NMR Spectroscopists.Journal of magnetic resonance open · 2022
    Article
  5. Chemical reviews · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Roman ZadorozhnyiDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States.
Sucharita SarkarDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States.
Caitlin M QuinnDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States.
Kaneil K ZadroznyDepartment of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, United States.
Barbie K Ganser-PornillosDepartment of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, United States.
Owen PornillosDepartment of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, United States.
Angela M GronenbornPittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.
Tatyana PolenovaDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, United States.

Funding

Technology Development 2: MAS NMR and dynamic nuclear polarization for HIV-1 structural biologyP50AI150481 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI GRONENBORN, ANGELA M. · 2019 to 2021
$14.1M
Molecular mechanisms of the CA-SP1 switch in HIV assembly and maturationR01AI129678 · NIAID · UNIVERSITY OF VIRGINIA · PI GANSER-PORNILLOS, BARBIE K., PORNILLOS, OWEN · 2017 to 2021
$2.3M
NIAID NIH HHS P50 AI150481NIAID NIH HHS R01 AI129678
6 · The paper itself

Abstract

Histidine residues play important structural and functional roles in proteins, such as serving as metal-binding ligands, mediating enzyme catalysis, and modulating proton channel activity. Many of these activities are modulated by the ionization state of the imidazole ring. Here we present a fast MAS NMR approach for the determination of protonation and tautomeric states of His at frequencies of 40-62 kHz. The experiments combine

Indexed as

Fast MAS NMRhistidine protonation stateMagic angle spinning (MAS)nuclear magnetic resonance (NMR) spectroscopysolid-state NMRtransferred echo double resonance (TEDOR)

Identifiers

PMID34957215
PMCPMC8703106

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.