ArticleFrontiers in cell and developmental biology2021
Single-Cell RNA Sequencing Revealed the Heterogeneity of Gonadal Primordial Germ Cells in Zebra Finch (
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 12 citations in OpenAlex.
- Comparative analysis of morphological atlas at different HH stages of embryonic development for SPF ducks and SPF chickens.Poultry science · 2026Article
- Transcriptional Profiling of Primordial Germ Cells During Chicken Embryonic Development.Veterinary sciences · 2026Article
- Efficient and Safe Knockout ofVeterinary sciences · 2026Article
- Manipulating primordial germ cells from chicken and other bird species.The Journal of reproduction and development · 2026Review
- In Vitro Culture of Avian Primordial Germ Cells: Established Methods and Future Directions.Biology · 2025Review
- Conserved functional features of natural killer cell subsets in chicken, human, and murine immune systems.iScience · 2025Article
- Single-nucleus transcriptional and chromatin accessible profiles reveal critical cell types and molecular architecture underlying chicken sex determination.Journal of advanced research · 2025Article
- Single-cell RNA sequencing reveals surface markers of primordial germ cells in chicken and zebra finch.Molecular genetics and genomics : MGG · 2024Article
- RNA sequencing analysis of sexual dimorphism in Japanese quail.Frontiers in veterinary science · 2024Article
- Single cell genomics as a transformative approach for aquaculture research and innovation.Reviews in aquaculture · 2023Review
- Review
- Finer resolution analysis of transcriptional programming during the active migration of chicken primordial germ cells.Computational and structural biotechnology journal · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primordial germ cells (PGCs) are undifferentiated gametes with heterogeneity, an evolutionarily conserved characteristic across various organisms. Although dynamic selection at the level of early germ cell populations is an important biological feature linked to fertility, the heterogeneity of PGCs in avian species has not been characterized. In this study, we sought to evaluate PGC heterogeneity in zebra finch using a single-cell RNA sequencing (scRNA-seq) approach. Using scRNA-seq of embryonic gonadal cells from male and female zebra finches at Hamburger and Hamilton (HH) stage 28, we annotated nine cell types from 20 cell clusters. We found that PGCs previously considered a single population can be separated into three subtypes showing differences in apoptosis, proliferation, and other biological processes. The three PGC subtypes were specifically enriched for genes showing expression patterns related to germness or pluripotency, suggesting functional differences in PGCs according to the three subtypes. Additionally, we discovered a novel biomarker,
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