Evidence map›Paper›PMID 34956617›Full record

ArticleToxicology research2021

LncRNA-ENST00000556926 regulates the proliferation, apoptosis and mRNA transcriptome of malignant-transformed BEAS-2B cells induced by coal tar pitch.

Yonghang Zhu, Zhongqiu Li, Weiguang Wang, Linhao Jing, Qi Yu, Zhenkai Li, Xu Chen, Jiatong Zhang, Peng Zhang, Feifei Feng and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicology research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Thioredoxin Domain Containing 5 (TXNDC5): Friend or Foe?Current issues in molecular biology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Yonghang ZhuDepartment of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Zhongqiu LiDepartment of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Weiguang WangDepartment of Disease Control and Prevention, Rizhao Center for Disease Control and Prevention, Rizhao, Shandong Province 450001, China.
Linhao JingCollege of Public Health, Zhengzhou University, Zhengzhou, Henan Province 276800, China.
Qi YuDepartment of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Zhenkai LiDepartment of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Xu ChenCollege of Public Health, Zhengzhou University, Zhengzhou, Henan Province 276800, China.
Jiatong ZhangDepartment of Disease Control and Prevention, Hospital of Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Peng ZhangDepartment of Bone and Soft Tissue Cancer, The Affiliated Cancer Hospital of Zhengzhou University (Henan Cancer Hospital), Zhengzhou, Henan Province 450001, China.
Feifei FengDepartment of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province 450001, China.
Qiao ZhangDepartment of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou, Henan Province 450001, China.ORCID https://orcid.org/0000-0002-1225-8661
Zhengzhou University · CNShandong Center for Disease Control and Prevention · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As a byproduct of coal tar distillation, coal tar pitch (CTP) has been proven to be carcinogenic to human. However, the mechanisms of lung cancer induced by CTP are still unclear. It has been shown that long non-coding RNAs (LncRNAs) play an important role in the development of human cancers. This study aims to investigate the effect of LncRNA-ENST00000556926 on malignant-transformed human bronchial epithelial (BAES-2B) cells induced by coal tar pitch extracts (CTPE). In this study, BEAS-2B cells were treated with 2.4 μg/ml of CTPE for 72 h and then passaged; and the cells were treated 4 times in the same procedure, then passaged until passage 30 (CTPE30). Cell counting kit-8 (CCK-8) assay was used to detect cell viability, then cell cycle and apoptosis were analyzed by flow cytometry, and transcriptome sequencing analysis was used to detect differentially expressed mRNAs after interference of ENST00000556926. The results indicated that the expression of ENST00000556926 in CTPE30 group was significantly higher compared with control group. Furthermore, after interfering the expression of ENST00000556926, cell viability was inhibited, and cell cycle was arrested while apoptosis of malignant-transformed BEAS-2B cells was promoted. Moreover, a total of 159 differentially expressed mRNAs were screened out after interference of ENST00000556926, including 62 up-regulated mRNAs and 97 down-regulated mRNAs. In addition, knockdown of ENST00000556926 decreased the expression of thioredoxin domain containing 5 (TXNDC5) and FOXD1. In conclusion, LncRNA-ENST00000556926 could regulate the proliferation, apoptosis and mRNA transcriptome of malignant-transformed BEAS-2B cells induced by CTP, which may provide a novel treatment strategy for lung cancer.

Indexed as

BEAS-2B cellscoal tar pitchENST00000556926FOXD1TXNDC5

Identifiers

PMID34956617
PMCPMC8692750
OpenAlexW3213037958

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.