ArticleJournal of cellular and molecular medicine2022
Studying the mechanism of sperm DNA damage caused by folate deficiency.
Article in Journal of cellular and molecular medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Gut microbiota and their role in male reproductive health.NPJ science of food · 2026Review
- The dual role of inflammatory microenvironment in modulating the effect of red blood cell folic acid on sperm DNA integrity: A retrospective study.Scientific reports · 2026Article
- MASLD: insights on the role of folate in hepatic lipid metabolism.Frontiers in nutrition · 2025Review
- Effect of chitosan on buck semen quality and semen plasma metabolites during low-temperature storage.Frontiers in veterinary science · 2025Article
- Folic acid and sperm quality improvement: insights from snRNA sequencing and RNA splicing mechanisms.Frontiers in nutrition · 2025Review
- Article
- The effect of red blood cell disorders on male fertility and reproductive health.Nature reviews. Urology · 2024Review
- Neural Tube Defects and Folate Deficiency: Is DNA Repair Defective?International journal of molecular sciences · 2023Review
- Investigation of Sperm and Seminal Plasma Candidate MicroRNAs of Bulls with Differing Fertility and In Silico Prediction of miRNA-mRNA Interaction Network of Reproductive Function.Animals : an open access journal from MDPI · 2022Article
- Studying the mechanism of sperm DNA damage caused by folate deficiency.Journal of cellular and molecular medicine · 2022Article
- Effect of iodoacetic acid on the reproductive system of male mice.Frontiers in pharmacology · 2022Article
- Advances in the diagnosis and treatment of oligoasthenozoospermia based on epigenetic regulation.Therapeutic advances in urologyReview
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm DNA injury is one of the common causes of male infertility. Folic acid deficiency would increase the methylation level of the important genes, including those involved in DNA double-strand break (DSB) repair pathway. In the early stages, we analysed the correlation between seminal plasma folic acid concentration and semen parameters in 157 infertility patients and 91 sperm donor volunteers, and found that there was a significant negative correlation between seminal folic acid concentration and sperm DNA Fragmentation Index (DFI; r = -0.495, p < 0.01). Then through reduced representation bisulphite sequencing, global DNA methylation of sperm of patients in the low folic acid group and the high folic acid group was analysed, it was found that the methylation level in Rad54 promoter region increased in the folic acid deficiency group compared with the normal folic acid group. Meanwhile, the results of animal model and spermatocyte line (GC-2) also found that folic acid deficiency can increase the methylation level in Rad54 promoter region, increased sperm DFI in mice, increased the expression of γ-H2AX, that is, DNA injury marker protein, and increased sensitivity of GC-2 to external damage and stimulation. The study indicates that the expression of Rad54 is downregulated by folic acid deficiency via DNA methylation. This may be one of the mechanisms of sperm DNA damage caused by folate deficiency.
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