ArticleJournal of radiation research2022
DNA strand break induction of aqueous plasmid DNA exposed to 30 MeV protons at ultra-high dose rate.
Article in Journal of radiation research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Examining the Occurrence of the FLASH Effect in Animal Models: A Systematic Review and Meta-Analysis of Ultra-High Dose Rate Proton or Carbon Ion Irradiation.Technology in cancer research & treatmentPooled it
- X‑ray FLASH RT inhibits lung cancer bone metastasis via activating CD4⁺ T-cell‑mediated antitumor immunity with modulation of Th17 cell response.Oncoimmunology · 2026Article
- Article
- Ultra-high dose rate dependent modeling of plasmid DNA damage with TOPAS-nBio.Physics in medicine and biology · 2026Article
- Oxygen Depletion in FLASH Particle Therapy: Effects of Linear Energy Transfer and Ion Track Structure.Antioxidants (Basel, Switzerland) · 2026Article
- Preliminary mechanistic study of mitochondrial function in intestinal protection mediated by high-energy X-ray FLASH radiotherapy.Radiation oncology (London, England) · 2026Article
- Biological effects of conventional and ultra high dose rate radiation in human cells.Scientific reports · 2026Article
- FLASH radiotherapy as an emerging paradigm in radioimmunotherapy: biological rationale, preclinical evidence, and translational roadmap.Frontiers in immunology · 2026Review
- Early molecular changes induced by FLASH irradiation in MCF10A and MDA-MB-231 breast cell lines.Frontiers in oncology · 2026Article
- Exploring the mechanisms of protective effect of high-energy X-ray FLASH radiotherapy on intestine through multi omics analysis.Radiation oncology (London, England) · 2025Article
- Effect of FLASH proton therapy on primary bronchial epithelial cell organoids.Clinical and translational radiation oncology · 2025Article
- Impact of Ultra-High-Dose-Rate Irradiation on DNA: Single-Strand Breaks and Base Damage.International journal of molecular sciences · 2025Article
- FLASH radiotherapy: mechanisms, nanotherapeutic strategy and future development.Nanoscale advances · 2025Review
- FLASH-RT for pulmonary protection: a comprehensive review of mechanisms, technological advances, and clinical translation.Frontiers in oncology · 2025Review
- Influence factor and mechanism of FLASH effect.Frontiers in oncology · 2025Review
- Potential effect of FLASH radiotherapy on testicular damage: a review of current evidence.Frontiers in oncology · 2025Review
- The oxygen puzzle in FLASH radiotherapy: A comprehensive review and experimental outlook.Clinical and translational radiation oncology · 2024Review
- Current views on mechanisms of the FLASH effect in cancer radiotherapy.National science review · 2024Review
- VHEE FLASH sparing effect measured at CLEAR, CERN with DNA damage of pBR322 plasmid as a biological endpoint.Scientific reports · 2024Article
- FLASH Radiotherapy: Mechanisms of Biological Effects and the Therapeutic Potential in Cancer.Biomolecules · 2024Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Radiation cancer therapy with ultra-high dose rate exposure, so called FLASH radiotherapy, appears to reduce normal tissue damage without compromising tumor response. The aim of this study was to clarify whether FLASH exposure of proton beam would be effective in reducing the DNA strand break induction. We applied a simple model system, pBR322 plasmid DNA in aqueous 1 × TE solution, where DNA single strand breaks (SSBs) and double strand breaks (DSBs) can be precisely quantified by gel electrophoresis. Plasmid DNA were exposed to 27.5 MeV protons in the conventional dose rate of 0.05 Gy/s (CONV) and ultra-high dose rate of 40 Gy/s (FLASH). With both dose rate, the kinetics of the SSB and DSB induction were proportional to absorbed dose. The SSB induction of FLASH was significantly less than CONV, which were 8.79 ± 0.14 (10-3 SSB per Gy per molecule) and 10.8 ± 0.68 (10-3 SSB per Gy per molecule), respectively. The DSB induction of FLASH was also slightly less than CONV, but difference was not significant. Altogether, 27.5 MeV proton beam at 40 Gy/s reduced SSB and not DSB, thus its effect may not be significant in reducing lethal DNA damage that become apparent in acute radiation effect.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.