ArticleOrganic & biomolecular chemistry2022
Anion binding to a cationic europium(III) probe enables the first real-time assay of heparan sulfotransferase activity.
Article in Organic & biomolecular chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Probing sulfotransferase binding and inhibition with synthetic PAPS analogs reveals the role of the 3'-phosphate and informs molecular tool design.RSC chemical biology · 2026Article
- Advances in lanthanide probes for selective anion recognition and sensing in water.Chemical science · 2026Review
- Protein binding regulates complex configuration: comparative analysis of three dynamically racemic europium(iii) complexes.RSC advances · 2026Article
- Unmasking the UV Photobleaching of β-Diketonate [Eu(BTFA)Inorganic chemistry · 2025Article
- Luminescent Lanthanides in Biorelated Applications: From Molecules to Nanoparticles and Diagnostic Probes to Therapeutics.Chemical reviews · 2025Review
- Polysaccharide sulfotransferases: the identification of putative sequences and respective functional characterisation.Essays in biochemistry · 2024Review
- Fluorescent ratiometric supramolecular tandem assays for phosphatase and phytase enzymes.Organic & biomolecular chemistry · 2024Article
- Luminescent Lanthanide Probes for Inorganic and Organic Phosphates.Chemistry, an Asian journal · 2022Review
- Fluorinated Phosphoadenosine 5'-Phosphosulfate Analogues for Continuous Sulfotransferase Activity Monitoring and Inhibitor Screening byACS chemical biology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sulfotransferases constitute a ubiquitous class of enzymes which are poorly understood due to the lack of a convenient tool for screening their activity. These enzymes use the anion PAPS (adenosine-3'-phosphate-5'-phosphosulfate) as a donor for a broad range of acceptor substrates, including carbohydrates, producing sulfated compounds and PAP (adenosine-3',5'-diphosphate) as a side product. We present a europium(III)-based probe that binds reversibly to both PAPS and PAP, producing a larger luminescence enhancement with the latter anion. We exploit this greater emission enhancement with PAP to demonstrate the first direct real-time assay of a heparan sulfate sulfotransferase using a multi-well plate format. The selective response of our probe towards PAP over structurally similar nucleoside phosphate anions, and over other anions, is investigated and discussed. This work opens the possibility of investigating more fully the roles played by this enzyme class in health and disease, including operationally simple inhibitor screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.