Padlock Probe-Based Generation of DNAzymes for the Colorimetric Detection of Antibiotic Resistance Genes.

Rick Conzemius, Ariadne Haunold, Ivan Barišić

Open access · goldAbstract read

In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact, top 87% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Smart colorimetric wound dressing for real-time infection detection and monitoring.Journal of biological engineering · 2026
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rick ConzemiusCenter for Health and Bioresources, Competence Unit Molecular Diagnostics, AIT Austrian Institute of Technology, Giefinggasse 4, A-1210 Vienna, Austria.ORCID 0000-0002-7116-8180

Ariadne HaunoldCenter for Health and Bioresources, Competence Unit Molecular Diagnostics, AIT Austrian Institute of Technology, Giefinggasse 4, A-1210 Vienna, Austria.

Ivan BarišićCenter for Health and Bioresources, Competence Unit Molecular Diagnostics, AIT Austrian Institute of Technology, Giefinggasse 4, A-1210 Vienna, Austria.ORCID 0000-0002-1301-6197

Austrian Institute of Technology · AT

Funding

European Commission 686647

6 · The paper itself

Abstract

The increasing emergence of multidrug- and pan-resistant pathogens requires rapid and cost-efficient diagnostic tools to contain their further spread in healthcare facilities and the environment. The currently established diagnostic technologies are of limited utility for efficient infection control measures because they are either cultivation-based and time-consuming or require sophisticated assays that are expensive. Furthermore, infectious diseases are unfortunately most problematic in countries with low-resource settings in their healthcare systems. In this study, we developed a cost-efficient detection technology that uses G-quadruplex DNAzymes to convert a chromogenic substrate resulting in a color change in the presence of antibiotic resistance genes. The assay is based on padlock probes capable of high-multiplex reactions and targets 27 clinically relevant antibiotic resistance genes associated with sepsis. In addition to an experimental proof-of-principle using synthetic target DNA, the assay was evaluated with multidrug-resistant clinical isolates.

Indexed as

Drug Resistance, BacterialAcinetobacter baumanniiBacteriaBacterial ProteinsBiosensing TechniquesColorimetryDNA, CatalyticDNA ProbesEnterobacter cloacaeEnterococcus faeciumG-QuadruplexesHumansKlebsiella pneumoniaeMultiplex Polymerase Chain ReactionNucleic Acid Amplification TechniquesProof of Concept StudyBacterial ProteinsDNA, CatalyticDNA Probesantibiotic resistancecolorimetric detectionDNAzymeG-quadruplexpadlock probe

Identifiers

PMID34948456

DOI10.3390/ijms222413654

PMCPMC8715668

OpenAlexW4200421008

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.