Evidence map›Paper›PMID 34948023›Full record

ArticleInternational journal of molecular sciences2021

Practical Considerations of Dissolved Oxygen Levels for Platelet Function under Hypoxia.

Branden Kusanto, Andrew Gordon, Leigh Naylor-Adamson, Lloyd Atkinson, Charlie Coupland, Zoe Booth, Yusra Ahmed, Isabel M Pires, Graeme J Stasiuk, Roger Sturmey and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Branden KusantoCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
Andrew GordonCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
Leigh Naylor-AdamsonCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
Lloyd AtkinsonCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
Charlie CouplandCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
Zoe BoothCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
Yusra AhmedCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
Isabel M PiresDepartment of Biomedical Sciences, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.ORCID 0000-0002-0417-2710
Graeme J StasiukDepartment of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging, King's College London, St. Thomas' Hospital, London SE1 7EH, UK.
Roger SturmeyCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.ORCID 0000-0003-4423-5058
Simon D J CalaminusCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
Mònica ArmanCentre for Atherothrombosis and Metabolic Disease, Hull York Medical School, Faculty of Health Sciences, University of Hull, Hull HU6 7RX, UK.
University of Hull · GBSt Thomas' Hospital · GB

Funding

British Heart Foundation FS/19/38/34441Medical Research Council MR/T002573/1
6 · The paper itself

Abstract

Investigating human platelet function in low-oxygen environments is important in multiple settings, including hypobaric hypoxia (e.g., high altitude), sea level hypoxia-related disease, and thrombus stability. These studies often involve drawing blood from which platelets are isolated and analysed at atmospheric conditions or re-exposed to low oxygen levels in hypoxia chambers before testing. However, it remains unknown how the in vitro handling of the samples itself changes their dissolved oxygen concentration, which might affect platelet function and experimental results. Here, we prepared healthy donor platelet-rich plasma and washed platelet (WP) suspensions and exposed them to 2% oxygen. We found that the use of hypoxia pre-equilibrated tubes, higher platelet concentrations (>2 × 10

Indexed as

AtmosphereBlood PlateletsCell HypoxiaHemostasisHumansOxygenPlatelet Function TestsPlatelet-Rich PlasmaOxygendissolved oxygen concentrationhypoxiain vitro assayplateletplatelet rich plasmawhole blood

Identifiers

PMID34948023
PMCPMC8703370
OpenAlexW4200088265

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.