ArticleThe Journal of cell biology1987
Electric field-induced redistribution and postfield relaxation of epidermal growth factor receptors on A431 cells.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts.Cell communication and signaling : CCS · 2025Article
- Uniform electric field generation in circular multi-well culture plates using polymeric inserts.Scientific reports · 2016Article
- Spontaneous and electric field-controlled front-rear polarization of human keratinocytes.Molecular biology of the cell · 2015Article
- In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation.Journal of neuroscience methods · 2014Article
- Evaluation of EGFR and RTK signaling in the electrotaxis of lung adenocarcinoma cells under direct-current electric field stimulation.PloS one · 2013Article
- Article
- The influence of electric fields on hippocampal neural progenitor cells.Stem cell reviews and reports · 2010Article
- Role of membrane potential in the regulation of cell proliferation and differentiation.Stem cell reviews and reports · 2009Review
- Epidermal growth factor receptor distribution during chemotactic responses.Molecular biology of the cell · 2000Article
- Electric field-directed cell motility involves up-regulated expression and asymmetric redistribution of the epidermal growth factor receptors and is enhanced by fibronectin and laminin.Molecular biology of the cell · 1999Article
- Electric field-directed fibroblast locomotion involves cell surface molecular reorganization and is calcium independent.The Journal of cell biology · 1994Article
- Electric fields induce curved growth of Enterobacter cloacae, Escherichia coli, and Bacillus subtilis cells: implications for mechanisms of galvanotropism and bacterial growth.Journal of bacteriology · 1994Article
- Cell shape-dependent rectification of surface receptor transport in a sinusoidal electric field.Biophysical journal · 1993Article
- Kinetics of epidermal growth factor/receptor binding on cells measured by total internal reflection/fluorescence recovery after photobleaching.Journal of fluorescence · 1991Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The lateral mobility of the epidermal growth factor (EGF) receptor in the plane of the plasma membrane of cultured A431 cells was investigated using direct and indirect fluorescent probes to measure the generation and relaxation of electric field-induced receptor asymmetry. A steady electric field of 15 V/cm for 30 min at 23 degrees C induced a redistribution of the unoccupied EGF receptor such that there was approximately a three-fold higher concentration of receptors at the cathode-facing pole. After termination of the field, the unoccupied receptors back diffused at 37 degrees C with a rate corresponding to a diffusion coefficient of 2.6-3.5 X 10(-10) cm2/s. No diffusion was detected at 4 degrees C. Formation of the hormone-receptor complex is known to induce receptor clustering and internalization. By inhibiting internalization with metabolic poisons, we were able to study the cell surface mobility of clusters of the hormone-receptor complex. The same degree of asymmetry was induced when the occupied receptor was exposed to an electric field and the rate of back diffusion of clusters of the hormone-receptor complex corresponded to a diffusion coefficient of 0.68-0.95 X 10(-10) cm2/s. Although the unoccupied receptor is somewhat more mobile than the hormone-receptor complex, it was still far less mobile than one would predict for an unconstrained protein imbedded in a phospholipid bilayer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.