Easy Expression and Purification of Fluorescent N-Terminal BCL11B CCHC Zinc Finger Domain.

Anne Susemihl, Felix Nagel, Piotr Grabarczyk, Christian A Schmidt, Mihaela Delcea

Open access · goldAbstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it

0cells of the map it votes in

4citing papers in PubMed

0.3field-weighted citation impact, top 43% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

Solution structure of the Z0 domain from transcription repressor BCL11A sheds light on the sequence properties of protein-binding zinc fingers.Protein science : a publication of the Protein Society · 2025
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Gene Networks and Pathways Involved in LPS-Induced Proliferative Response of Bovine Endometrial Epithelial Cells.Genes · 2022
Article
Structural Basis of the Pancreatitis-Associated Autoproteolytic Failsafe Mechanism in Human Anionic Trypsin.Journal of inflammation research · 2022
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Anne SusemihlDepartment of Biophysical Chemistry, Institute of Biochemistry, University of Greifswald, 17489 Greifswald, Germany.ORCID 0000-0002-6363-9325

Felix NagelDepartment of Biophysical Chemistry, Institute of Biochemistry, University of Greifswald, 17489 Greifswald, Germany.ORCID 0000-0003-3456-7075

Piotr GrabarczykDepartment of Hematology and Oncology, Internal Medicine C, University of Greifswald, 17489 Greifswald, Germany.

Christian A SchmidtDepartment of Hematology and Oncology, Internal Medicine C, University of Greifswald, 17489 Greifswald, Germany.

Mihaela DelceaDepartment of Biophysical Chemistry, Institute of Biochemistry, University of Greifswald, 17489 Greifswald, Germany.

Universität Greifswald · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zinc finger proteins play pivotal roles in health and disease and exert critical functions in various cellular processes. A majority of zinc finger proteins bind DNA and act as transcription factors. B-cell lymphoma/leukemia 11B (BCL11B) represents one member of the large family of zinc finger proteins. The N-terminal domain of BCL11B was shown to be crucial for BCL11B to exert its proper function by homodimerization. Here, we describe an easy and fast preparation protocol to yield the fluorescently tagged protein of the recombinant N-terminal BCL11B zinc finger domain (BCL11B

Indexed as

Gene ExpressionGreen Fluorescent ProteinsRecombinant Fusion ProteinsRepressor ProteinsTumor Suppressor ProteinsEscherichia coliHumansProtein DomainsZinc FingersBCL11B protein, humanGreen Fluorescent ProteinsRecombinant Fusion ProteinsRepressor ProteinsTumor Suppressor ProteinsBCL11BFREThomodimerizationprotein expression and purificationzinc finger

Identifiers

PMID34946663

DOI10.3390/molecules26247576

PMCPMC8708588

OpenAlexW4200148549

What OpenQuestion holds

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LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.