Evidence map›Paper›PMID 34946019›Full record

ArticleMicroorganisms2021

Antimicrobial Activity of Chemically and Biologically Treated Chitosan Prepared from Black Soldier Fly (

Mevin Kiprotich Lagat, Samuel Were, Francis Ndwigah, Violah Jepkogei Kemboi, Carolyne Kipkoech, Chrysantus Mbi Tanga

Open access · goldAbstract read
In one paragraph

Article in Microorganisms, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 72 citations in OpenAlex.

  1. Animals : an open access journal from MDPI · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Mevin Kiprotich LagatDepartment of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-02000, Kenya.
Samuel WereDepartment of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-02000, Kenya.
Francis NdwigahDepartment of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-02000, Kenya.
Violah Jepkogei KemboiDepartment of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-02000, Kenya.
Carolyne KipkoechDepartment of Food and Nutritional Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-02000, Kenya.
Chrysantus Mbi TangaInternational Centre of Insect Physiology and Ecology (icipe), Nairobi P.O. Box 30772-00100, Kenya.ORCID 0000-0002-5788-7920
Jomo Kenyatta University of Agriculture and Technology · KEInternational Centre of Insect Physiology and Ecology · KE

Funding

Bill & Melinda Gates Foundation INV-032416CAP-Africa RAF-3058 KEN-18/0005INSFEED 108866-001SiPFeed 2018 FOD 009
6 · The paper itself

Abstract

Globally, the broad-spectrum antimicrobial activity of chitin and chitosan has been widely documented. However, very little research attention has focused on chitin and chitosan extracted from black soldier fly pupal exuviae, which are abundantly present as byproducts from insect-farming enterprises. This study presents the first comparative analysis of chemical and biological extraction of chitin and chitosan from BSF pupal exuviae. The antibacterial activity of chitosan was also evaluated. For chemical extraction, demineralization and deproteinization were carried out using 1 M hydrochloric acid at 100 °C for 2 h and 1 M NaOH for 4 h at 100 °C, respectively. Biological chitin extraction was carried out by protease-producing bacteria and lactic-acid-producing bacteria for protein and mineral removal, respectively. The extracted chitin was converted to chitosan via deacetylation using 40% NaOH for 8 h at 100 °C. Chitin characterization was done using FTIR spectroscopy, while the antimicrobial properties were determined using the disc diffusion method. Chemical and biological extraction gave a chitin yield of 10.18% and 11.85%, respectively. A maximum chitosan yield of 6.58% was achieved via chemical treatment. From the FTIR results, biological and chemical chitin showed characteristic chitin peaks at 1650 and 1550 cm

Indexed as

antimicrobial activity keywordchitinchitosanHermetia illucensinsectspupal exuviae

Identifiers

PMID34946019
PMCPMC8706517
OpenAlexW3214793257

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.