Evidence map›Paper›PMID 34943846›Full record

ArticleCells2021

Design and Construction of a Chamber Enabling the Observation of Living Cells in the Field of a Constant Magnetic Force.

Daniel Dziob, Jakub Ramian, Jan Ramian, Bartosz Lisowski, Jadwiga Laska

Abstract read
In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Daniel DziobFaculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, Poland.ORCID 0000-0003-2065-600X
Jakub RamianFaculty of Mechanical engineering and Robotics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland.
Jan RamianFaculty of Medical Sciences in Katowice, Medical University of Silesia, Poniatowskiego 15, 40-055 Katowice, Poland.
Bartosz LisowskiFaculty of Medicine, Jagiellonian University Medical College, św. Łazarza 16, 31-530 Kraków, Poland.ORCID 0000-0003-1444-4924
Jadwiga LaskaFaculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland.ORCID 0000-0002-7230-3882

Funding

National Science Center K/PBM/000616
6 · The paper itself

Abstract

The aim of the work was to design and construct a microscopic stage that enables the observation of biological cells in a magnetic field with a constant magnetic force. Regarding the requirements for biological observations in the magnetic field, construction was based on the standard automatic stage of an optical microscope ZEISS Axio Observer, and the main challenge was to design a set of magnets which were the source of a field in which the magnetic force was constant in the observation zone. Another challenge was to design a magnet arrangement producing a weak magnetic field to manipulate the cells without harming them. The Halbach array of magnets was constructed using permanent cubic neodymium magnets mounted on a 3D printed polymer ring. Four sets of magnets were used, differing in their dimensions, namely, 20, 15, 12, and 10 mm. The polymer rings were designed to resist magnetic forces and to keep their shape undisturbed when working under biological conditions. To check the usability of the constructs, experiments with magnetic microparticles were executed. Magnetic microparticles were placed under the microscope and their movement was observed to find the acting magnetic force.

Indexed as

Magnetic FieldsCell SurvivalEquipment DesignImaging, Three-DimensionalMagneticsPrinting, Three-DimensionalReproducibility of ResultsTemperatureHalbach magnetliving cell observationmagnetic fieldoptical microscopy

Identifiers

PMID34943846
PMCPMC8699672

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.