ArticleThe Science of the total environment2022
Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?
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In one paragraph
Article in The Science of the total environment, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 21 papers.
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21citing papers in PubMed
2.0field-weighted citation impact, top 11% of its field
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
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Corrections and comments
- Retraction · 2024-11-17Date of Article and/or Notice Unknown · Compromised Peer Review · Investigation by Journal/Publisher · Unreliable Results and/or Conclusions · · date of retraction unknown, html page overwrite, date estimated from notifications; separate notice issued November 27, 2024.
- Retracted
Authors and funding
64 authors at 12 institutions in 3 countries.
Bianca H Ventura FernandesLaboratório de Controle Genético e Sanitário, Diretoria Técnica de Apoio ao Ensino e Pesquisa, Faculdade de Medicina da Universidade de São Paulo, Brazil.
Natália Martins FeitosaLaboratório Integrado de Biociências Translacionais (LIBT), Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro (UFRJ), Macaé, RJ, Brazil.
Ana Paula BarbosaDepartment of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Camila Gasque BomfimDepartment of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Anali M B GarniqueDepartment of Cell Biology, Institute of Biomedical Sciences, University of São Paulo, Brazil.
Ivana F RosaReproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
Maira S RodriguesReproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
Lucas B DorettoReproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
Daniel F CostaReproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
Bruno Camargo-Dos-SantosDepartment of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
Gabrielli A FrancoDepartment of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
João Favero NetoDepartment of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
Juliana Sartori LunardiDepartment of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
Marina Sanson BellotDepartment of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
Nina Pacheco Capelini AlvesDepartment of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
Camila C CostaDepartment of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil.
Mayumi F AracatiDepartment of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil.
Letícia F RodriguesDepartment of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil.
Camila C CostaDepartment of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil.
Rafaela Hemily CiriloDepartment of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
Raul Marcelino ColagrandeDepartment of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
Francisco I F GomesDepartment of Pharmacology, Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of São Paulo, Brazil.
Rafael T NakajimaReproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
Marco A A BeloBrasil University, Descalvado, São Paulo, Brazil.
Percília Cardoso GiaquintoUniversidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências - Departamento de Fisiologia, São Paulo, Brazil.
Susana Luporini de OliveiraUniversidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, Brazil.
Silas Fernandes EtoPostgraduate Program in Health Sciences, PROCISA, Federal University of Roraima, Brazil.
Dayanne Carla FernandesImmunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
Wilson G ManriqueAquaculture Health Research and Extension Group, GRUPESA, Aquaculture Health Laboratory, LABSA, Department of Veterinary Medicine, Federal University of Rondônia, Rolim de Moura campus, Rondônia, Brazil.
Gabriel CondeDepartment of Preventive Veterinary Medicine, São Paulo State University, Jaboticabal, Brazil.
Roberta R C RosalesDepartment of Cell and Molecular Biology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
Iris TodeschiniDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
Ilo RiveroPontifícia Universidade Católica de Minas Gerais, Brazil.
Edgar LlontopDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
Germán G SgroDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil; Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Gabriel Umaji OkaDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
Natalia Fernanda BuenoDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
Fausto K FerrarisDepartment of Pharmacology and Toxicology, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil.
Mariana T Q de MagalhãesDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Renata J MedeirosLaboratory of Physiology, INCQS/Fiocruz Zebrafish Facility, Departament of Pharmacology and Toxicology, National Institute for Quality Control in Health, Brasil.
Juliana M Mendonça-GomesTransplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil.
Mara Souza JunqueiraCenter for Translational Research in Oncology, Cancer Institute of the State of Sao Paulo, Faculty of Medicine, University of São Paulo, Sao Paulo, Brazil.
Kátia ConceiçãoLaboratory of Peptide Biochemistry, Federal University of São Paulo, Brazil.
Leticia Gomes de PontesLaboratory of Human Immunology, Department Immunology, Institute Biomedical Sciences, University São Paulo, Sao Paulo, Brazil.
Antonio Condino-NetoLaboratory of Human Immunology, Department Immunology, Institute Biomedical Sciences, University São Paulo, Sao Paulo, Brazil.
Andrea C PerezDepartment of Pharmacology, Universidade Federal de Minas Gerais, Brazil.
Leonardo J G BarcellosGraduate Program of Pharmacology, Federal University of Santa Maria, Brazil; Laboratory of Fish Physiology, Graduate Program of Bioexperimentation and of Environmental Sciences, University of Passo Fundo, Brazil.
José Dias Correa JúniorLaboratório do Estudo da Interação Químico Biológica e da Reprodução Animal, LIQBRA, Bloco O3,174, Brazil; Departamento de Morfologia Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
Erick Gustavo DorlassDepartment of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Brazil.
Niels O S CamaraTransplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil.
Edison Luiz DurigonDepartment of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Fernando Q CunhaDepartment of Pharmacology, Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
Rafael H NóbregaReproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
Glaucia M Machado-SantelliDepartment of Cell Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Chuck S FarahDepartamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
Flavio P VerasCenter of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, Sao Paulo, Brazil; Department of Pharmacology, Ribeirao Preto Medical School, University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
Jorge Galindo-VillegasFaculty of Biosciences and Aquaculture, Nord University, 8049 Bodo, Norway.
Letícia V Costa-LotufoDepartment of Pharmacology, Institute of Biomedical Sciences, Universidade de São Paulo, Brazil.
Thiago M CunhaCenter of Research in Inflammatory Diseases, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, Sao Paulo, Brazil; Department of Pharmacology, Ribeirao Preto Medical School, University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
Roger ChammasCentro de Investigação Translacional em Oncologia, Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina da Universidade de São Paulo, Brazil.
Luciani R CarvalhoDisciplina de Endocrinologia do Departamento de Clinica Medica e Laboratório de Hormônios e Genética Molecular, LIM 42, Brazil.
Cristiane R GuzzoDepartment of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
Guilherme MalafaiaBiological Research Laboratory, Goiano Federal Institute, Urutaí Campus, Brazil. Electronic address: guilhermeifgoiano@gmail.com.
Ives Charlie-SilvaDepartment of Pharmacology, Institute of Biomedical Sciences, Universidade de São Paulo, Brazil. Electronic address: charliesilva4@hotmail.com.
Universidade Estadual Paulista (Unesp) · BRUniversidade de São Paulo · BRUniversidade Federal de Minas Gerais · BRInstituto Federal Goiano · BRInstituto do Câncer do Estado de São Paulo · BRNord University · NOUniversidade Federal de Santa Maria · BRFundação Oswaldo Cruz · BRUniversidade Brasil · BRUniversidade de Passo Fundo · BRUniversidade Federal de São Paulo · BRUniversidade Federal do Rio de Janeiro · BR
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Danio rerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), which corresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney, ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasons for the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6% of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highly toxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccine responses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assess the harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research.
Indexed as
COVID-19Spike Glycoprotein, CoronavirusAnimalsFemaleHumansSARS-CoV-2ZebrafishSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Acute respiratory syndromeCoronavirusDanio rerioEnvironmental impactsInfection diseases
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