ArticlePLoS genetics2021
Loss of YhcB results in dysregulation of coordinated peptidoglycan, LPS and phospholipid synthesis during Escherichia coli cell growth.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
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- Essential Role of LapD in the Absence of Cardiolipins.International journal of molecular sciences · 2026Article
- Coordinated Biosynthesis of Essential Cell Envelope Components: Lipopolysaccharide and Fatty Acids Requires LapD, Acyl Carrier Protein, and Fully Hexaacylated Lipid A.International journal of molecular sciences · 2025Article
- Decoding gene essentiality inMicrobiology spectrum · 2025Article
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- Transposon mutagenesis screen ineLife · 2024Article
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- Co-ordinated assembly of the multilayered cell envelope of Gram-negative bacteria.Current opinion in microbiology · 2024Review
- NhaA facilitates the maintenance of bacterial envelope integrity and the evasion of complement attack contributing to extraintestinal pathogenicInfection and immunity · 2023Article
- Microbial Primer: Transposon directed insertion site sequencing (TraDIS): A high throughput method for linking genotype to phenotype.Microbiology (Reading, England) · 2023Article
- Suppressors ofInternational journal of molecular sciences · 2023Article
- Cross-talk between phospholipid synthesis and peptidoglycan expansion by a cell wall hydrolase.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Preparation of Hybrid Nanopigments with Excellent Environmental Stability, Antibacterial and Antioxidant Properties Based onNanomaterials (Basel, Switzerland) · 2023Article
- The divisome but not the elongasome organizes capsule synthesis in Streptococcus pneumoniae.Nature communications · 2023Article
- A multiomic approach to defining the essential genome of the globally important pathogen Corynebacterium diphtheriae.PLoS genetics · 2023Article
- Critical role of the RpoE stress response pathway in polymyxin resistance of Escherichia coli.The Journal of antimicrobial chemotherapy · 2023Article
- LI-Detector: a Method for Curating Ordered Gene-Replacement Libraries.Microbiology spectrum · 2022Article
- A New Factor LapD Is Required for the Regulation of LpxC Amounts and Lipopolysaccharide Trafficking.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
26 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cell envelope is essential for viability in all domains of life. It retains enzymes and substrates within a confined space while providing a protective barrier to the external environment. Destabilising the envelope of bacterial pathogens is a common strategy employed by antimicrobial treatment. However, even in one of the best studied organisms, Escherichia coli, there remain gaps in our understanding of how the synthesis of the successive layers of the cell envelope are coordinated during growth and cell division. Here, we used a whole-genome phenotypic screen to identify mutants with a defective cell envelope. We report that loss of yhcB, a conserved gene of unknown function, results in loss of envelope stability, increased cell permeability and dysregulated control of cell size. Using whole genome transposon mutagenesis strategies, we report the comprehensive genetic interaction network of yhcB, revealing all genes with a synthetic negative and a synthetic positive relationship. These genes include those previously reported to have a role in cell envelope biogenesis. Surprisingly, we identified genes previously annotated as essential that became non-essential in a ΔyhcB background. Subsequent analyses suggest that YhcB functions at the junction of several envelope biosynthetic pathways coordinating the spatiotemporal growth of the cell, highlighting YhcB as an as yet unexplored antimicrobial target.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.