ArticlePLoS biology2021
Meiotic Cas9 expression mediates gene conversion in the male and female mouse germline.
Article in PLoS biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- The nanosd integral gene drive enables population modification of the malaria vector Anopheles gambiae.G3 (Bethesda, Md.) · 2026Article
- Female- or male-biased offspring generated by targeted distortion of sex chromosome transmission in the mouse.Genetics · 2026Article
- Finding the perfect promoter for Cas9 in homing gene drives using single cell transcriptome data.Nature communications · 2026Article
- Y-linked editors for invasive rodent control.Molecular biology and evolution · 2026Article
- The LAST Mile: Evaluating Genetic Biocontrol as a Supplemental Tool for Eradicating Invasive Rodents on Islands.Evolutionary applications · 2026Article
- Synthetic homing endonuclease gene drives to revolutionise Aedes aegypti biocontrol - game changer or pipe dream?Current opinion in insect science · 2025Review
- Maximising Eradication Potential of Rat Gene Drives Using a Two-Target Homing Rescue Strategy: Spatial Modelling of Empirical Data.Molecular ecology · 2025Article
- Engineering drive-selection balance for localized population suppression with neutral dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Investigating the potential of X chromosome shredding for mouse genetic biocontrol.Scientific reports · 2024Article
- Performance characteristics allow for confinement of a CRISPR toxin-antidote gene drive for population suppression in a reaction-diffusion model.Proceedings. Biological sciences · 2024Article
- Germline Cas9 promoters with improved performance for homing gene drive.Nature communications · 2024Article
- A framework for identifying fertility gene targets for mammalian pest control.Molecular ecology resources · 2024Article
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- Next-generation CRISPR gene-drive systems using Cas12a nuclease.Nature communications · 2023Article
- Harnessing Wolbachia cytoplasmic incompatibility alleles for confined gene drive: A modeling study.PLoS genetics · 2023Article
- Gene drive-mediated population elimination for biodiversity conservation. When you come to a fork in the road, take it.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias.Nature communications · 2022Article
- Leveraging a natural murine meiotic drive to suppress invasive populations.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Daisy-chain gene drives: The role of low cut-rate, resistance mutations, and maternal deposition.PLoS genetics · 2022Article
- Active genetics comes alive: Exploring the broad applications of CRISPR-based selfish genetic elements (or gene-drives): Exploring the broad applications of CRISPR-based selfish genetic elements (or gene-drives).BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Highly efficient gene conversion systems have the potential to facilitate the study of complex genetic traits using laboratory mice and, if implemented as a "gene drive," to limit loss of biodiversity and disease transmission caused by wild rodent populations. We previously showed that such a system of gene conversion from heterozygous to homozygous after a sequence targeted CRISPR/Cas9 double-strand DNA break (DSB) is feasible in the female mouse germline. In the male germline, however, all DSBs were instead repaired by end joining (EJ) mechanisms to form an "insertion/deletion" (indel) mutation. These observations suggested that timing Cas9 expression to coincide with meiosis I is critical to favor conditions when homologous chromosomes are aligned and interchromosomal homology-directed repair (HDR) mechanisms predominate. Here, using a Cas9 knock-in allele at the Spo11 locus, we show that meiotic expression of Cas9 does indeed mediate gene conversion in the male as well as in the female germline. However, the low frequency of both HDR and indel mutation in both male and female germlines suggests that Cas9 may be expressed from the Spo11 locus at levels too low for efficient DSB formation. We suggest that more robust Cas9 expression initiated during early meiosis I may improve the efficiency of gene conversion and further increase the rate of "super-mendelian" inheritance from both male and female mice.
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