ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2022
Sensitization of primary cultures from rat dorsal root ganglia with lipopolysaccharide (LPS) requires a robust inflammatory response.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 4 citations in OpenAlex.
- Transient Receptor Potential Melastatin 8 Plays a Key Role in Dextran Sulfate Sodium-Induced Murine Colitis via Regulating Extrinsic Sensory Neurons and Enteric Macrophage Secretory Activities.Neurogastroenterology and motility · 2026Article
- Beyond development: LINX/Islr2 expression and TrkA interaction in the adult nervous system under neuroinflammatory challenge.Frontiers in molecular neuroscience · 2026Article
- Pain research in a petri dish? Advantages and limitations of neuro-glial primary cell cultures from structures of the nociceptive system.Brain, behavior, & immunity - health · 2024Article
- Systemic Lipopolysaccharide Challenge Induces Inflammatory Changes in Rat Dorsal Root Ganglia: An Ex Vivo Study.International journal of molecular sciences · 2022Article
- Expression of adipokines and adipocytokines by epidural adipose tissue in cauda equina syndrome in dogs.Journal of veterinary internal medicine · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveWe investigated whether it is possible to induce a state of "LPS-sensitization" in neurons of primary cultures from rat dorsal root ganglia by pre-treatment with ultra-low doses of LPS.
methodsDRG primary cultures were pre-treated with low to ultra-low doses of LPS (0.001-0.1 µg/ml) for 18 h, followed by a short-term stimulation with a higher LPS-dose (10 µg/ml for 2 h). TNF-α in the supernatants was measured as a sensitive read out. Using the fura-2 340/380 nm ratio imaging technique, we further investigated the capsaicin-evoked Ca
resultsRelease of TNF-α evoked by stimulation with 10 µg/ml LPS into the supernatant was not significantly modified by pre-exposure to low to ultra-low LPS-doses. Capsaicin-evoked Ca
conclusionUltra-low doses of LPS, which per se do not evoke a detectable inflammatory response, are not sufficient to sensitize neurons (Ca
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Registered trials
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