Evidence map›Paper›PMID 34940247›Full record

ArticleBiosensors2021

A Pencil-Lead Immunosensor for the Rapid Electrochemical Measurement of Anti-Diphtheria Toxin Antibodies.

Wilson A Ameku, Vanessa N Ataide, Eric T Costa, Larissa R Gomes, Paloma Napoleão-Pêgo, David William Provance, Thiago R L C Paixão, Maiara O Salles, Salvatore Giovanni De-Simone

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Wilson A AmekuOswaldo Cruz Foundation (FIOCRUZ), Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Populations Diseases (INCT-IDPN), Rio de Janeiro 21040-900, Brazil.ORCID 0000-0002-5850-2033
Vanessa N AtaideElectronic Languages and Electrochemical Sensors Laboratory, Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000, Brazil.
Eric T CostaElectronic Languages and Electrochemical Sensors Laboratory, Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000, Brazil.
Larissa R GomesOswaldo Cruz Foundation (FIOCRUZ), Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Populations Diseases (INCT-IDPN), Rio de Janeiro 21040-900, Brazil.ORCID 0000-0001-7194-0858
Paloma Napoleão-PêgoOswaldo Cruz Foundation (FIOCRUZ), Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Populations Diseases (INCT-IDPN), Rio de Janeiro 21040-900, Brazil.
David William ProvanceOswaldo Cruz Foundation (FIOCRUZ), Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Populations Diseases (INCT-IDPN), Rio de Janeiro 21040-900, Brazil.ORCID 0000-0002-9824-0672
Thiago R L C PaixãoElectronic Languages and Electrochemical Sensors Laboratory, Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000, Brazil.ORCID 0000-0003-0375-4513
Maiara O SallesInstitute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Brazil.ORCID 0000-0002-0581-2038
Salvatore Giovanni De-SimoneOswaldo Cruz Foundation (FIOCRUZ), Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Populations Diseases (INCT-IDPN), Rio de Janeiro 21040-900, Brazil.ORCID 0000-0002-2172-656X
Fundação Oswaldo Cruz · BRUniversidade de São Paulo · BRUniversidade Federal do Rio de Janeiro · BRUniversidade Federal Fluminense · BR

Funding

Oswaldo Cruz Foundation INOVA (#VPPCB-007FIO-18-2-27 21, #VPPIS-005FIO- 20-2-51)
6 · The paper itself

Abstract

Diphtheria is a vaccine-preventable disease, yet immunization can wane over time to non-protective levels. We have developed a low-cost, miniaturized electroanalytical biosensor to quantify anti-diphtheria toxin (DTx) immunoglobulin G (anti-DTx IgG) antibody to minimize the risk for localized outbreaks. Two epitopes specific to DTx and recognized by antibodies generated post-vaccination were selected to create a bi-epitope peptide, biEP, by synthesizing the epitopes in tandem. The biEP peptide was conjugated to the surface of a pencil-lead electrode (PLE) integrated into a portable electrode holder. Captured anti-DTx IgG was measured by square wave voltammetry from the generation of hydroquinone (HQ) from the resulting immunocomplex. The performance of the biEP reagent presented high selectivity and specificity for DTx. Under the optimized working conditions, a logarithmic calibration curve showed good linearity over the concentration range of 10

Indexed as

Biosensing TechniquesDiphtheria ToxinEnzyme-Linked Immunosorbent AssayEpitopesImmunoassayImmunoglobulin GDiphtheria ToxinEpitopesImmunoglobulin GB-cell epitopebiosensordiphtheriaelectrochemistrypoint-of-caresquare wave voltammetry

Identifiers

PMID34940247
PMCPMC8699316
OpenAlexW3216236441

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.