ArticleNucleic acids research2022
Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 8 citations in OpenAlex.
- Impact of rapidly cleaving embryos on blastocyst formation and single-blastocyst transfer outcomes.Frontiers in endocrinology · 2026Article
- Genome instability in mammalian embryos implications for genome editing, development, and evolution.Current opinion in genetics & development · 2025Review
- DNA polymerase κ participates in early S-phase DNA replication in human cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Studying Translesion DNA Synthesis Using Xenopus In Vitro Systems.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Detection of endogenous translesion DNA synthesis in single mammalian cells.Cell reports methods · 2023Article
- Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.International journal of molecular sciences · 2021Review
- Green tea extract increases the quality and reduced DNA mutation of post-thawed Kacang buck sperm.Heliyon · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In early embryogenesis of fast cleaving embryos, DNA synthesis is short and surveillance mechanisms preserving genome integrity are inefficient, implying the possible generation of mutations. We have analyzed mutagenesis in Xenopus laevis and Drosophila melanogaster early embryos. We report the occurrence of a high mutation rate in Xenopus and show that it is dependent upon the translesion DNA synthesis (TLS) master regulator Rad18. Unexpectedly, we observed a homology-directed repair contribution of Rad18 in reducing the mutation load. Genetic invalidation of TLS in the pre-blastoderm Drosophila embryo resulted in reduction of both the hatching rate and single-nucleotide variations on pericentromeric heterochromatin in adult flies. Altogether, these findings indicate that during very early Xenopus and Drosophila embryos TLS strongly contributes to the high mutation rate. This may constitute a previously unforeseen source of genetic diversity contributing to the polymorphisms of each individual with implications for genome evolution and species adaptation.
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