ArticleScience China. Life sciences2022
Efficient de novo assembly and modification of large DNA fragments.
Article in Science China. Life sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Article
- Unexpected Diversity in Gene Clusters Encoding Formate Hydrogenlyase Complex Machinery in Vibrionaceae Correlated to Fermentative Hydrogen Production.Current microbiology · 2025Article
- Recent technology advancements in large-scale DNA assembly.Quantitative biology (Beijing, China) · 2025Article
- Optimization of PCA Error Correction Conditions to Improve Efficiency of Virus Genome De Novo Synthesis.International journal of molecular sciences · 2024Article
- A seven-sex species recognizes self and non-self mating-type via a novel protein complex.eLife · 2024Article
- A designer synthetic chromosome fragment functions in moss.Nature plants · 2024Article
- Crucial amino acids identified in Δ12 fatty acid desaturases related to linoleic acid production inFrontiers in plant science · 2024Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Towards Plant Synthetic Genomics.Biodesign research · 2023Article
- Digital Noah's Ark: last chance to save the endangered species.Science China. Life sciences · 2022Article
- Studying bona fide SARS-CoV-2 biology in a BSL-2 biosafety environment using a split-virus-genome system.Science China. Life sciences · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic genomics has provided new bottom-up platforms for the functional study of viral and microbial genomes. The construction of the large, gigabase (Gb)-sized genomes of higher organisms will deepen our understanding of genetic blueprints significantly. But for the synthesis and assembly of such large-scale genomes, the development of new or expanded methods is required. In this study, we develop an efficient pipeline for the construction of large DNA fragments sized 100 kilobases (kb) or above from scratches and describe an efficient method for "scar-free" engineering of the assembled sequences. Our method, therefore, should provide a standard framework for producing long DNA molecules, which are critical materials for synthetic genomics and metabolic engineering.
Indexed as
Identifiers
34939159What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.