ArticleApplied microbiology and biotechnology2022
Development and application of a colloidal-gold dual immunochromatography strip for detecting African swine fever virus antibodies.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Rapid and visual dual-color lateral flow immunoassay for african swine fever virus antibody detection.Applied microbiology and biotechnology · 2026Article
- A quantum dots dual immunochromatography strip for differential detection of high and low virulence ASFV antibodies.Microbiology spectrum · 2026Article
- Review
- Development of a colloidal gold immunochromatographic strip to detect equine infectious anemia virus.Virology journal · 2025Article
- Rapid detection of African swine fever virus by a blue latex microsphere immunochromatographic strip.AMB Express · 2025Article
- Development of a smartphone camera-based chemiluminescent lateral flow immunoassay for detecting African swine fever virus antibodies.Frontiers in microbiology · 2025Article
- On-Site Rapid and Specific Detection of Novel Emerging Goose Astrovirus Using a Nanobody-Based Colloidal Gold Immunochromatographic Strip.Transboundary and emerging diseases · 2025Article
- Development of a colloidal gold-based immunochromatographic strip for rapid detection ofFrontiers in microbiology · 2025Article
- Technologies for detecting biological risk factors in agricultural products and their applications.Current research in food science · 2025Review
- Pathogenicity and virulence of African swine fever virus.Virulence · 2024Review
- Development and evaluation of a test strip for the rapid detection of antibody against equine infectious anemia virus.Applied microbiology and biotechnology · 2024Article
- Development of a fully automated chemiluminescent immunoassay for the quantitative and qualitative detection of antibodies against African swine fever virus p72.Microbiology spectrum · 2024Article
- African Swine Fever Diagnosis in Africa: Challenges and Opportunities.Pathogens (Basel, Switzerland) · 2024Review
- Article
- Advanced Strategies for Developing Vaccines and Diagnostic Tools for African Swine Fever.Viruses · 2023Review
- Colloidal gold and fluorescent immunochromatographic test strips for canine parvovirus detection.Applied microbiology and biotechnology · 2023Article
- Identification of p72 epitopes of African swine fever virus and preliminary application.Frontiers in microbiology · 2023Article
- Identification of a novel B-cell epitope of the African swine fever virus p34 protein and development of an indirect ELISA for the detection of serum antibodies.Frontiers in microbiology · 2023Article
- An Improved Immunochromatographic Strip Based on Plant-Derived E2 for Detection of Antibodies against Classical Swine Fever Virus.Microbiology spectrum · 2022Article
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
Abstract
African swine fever (ASF) is an acute and highly contagious infectious disease caused by the African swine fever virus (ASFV). Currently, there is no vaccine against ASF worldwide, and no effective treatment measures are available. For this reason, developing a simple, rapid, specific, and sensitive serological detection method for ASFV antibodies is crucial for the prevention and control of ASF. In this study, a 1:1 mixture of gold-labeled p30 and p72 probes was used as the gold-labeled antigen. The p30 and p72 proteins and their monoclonal antibodies were coated on a nitrocellulose membrane (NC) as a test (T) line and control (C) line, respectively. A colloidal-gold dual immunochromatography strip (ICS) for ASFV p30 and p72 protein antibodies was established. The results showed that the colloidal-gold dual ICS could specifically detect ASFV antibodies within 5-10 min. There was no cross-reaction after testing healthy pig serum; porcine reproductive and respiratory syndrome virus (PRRSV), foot-and-mouth disease type A virus (FMDV-A), foot-and-mouth disease type O virus (FMDV-O), porcine circovirus type 2 (PCV-2), and classical swine fever virus (CSFV) positive sera. A positive result was obtained only for the positive control P1. The sensitivity of the test strips was 1:256, which was equivalent to that of commercially ELISA kits. Their coincidence rate with the two commercial ASFV ELISA antibodies detection kits was higher than 98%. The test strips were stably stored at 18-25 °C and 4 °C for 4 and 6 months, respectively. The colloidal-gold dual ICS prepared in this study had high sensitivity and specificity and were characterized by rapid detection, simple operation, and easy interpretation of results. Therefore, they are of great significance to diagnose, prevent, and control African swine fever. KEY POINTS: • We establish an antibody detection that is quick and can monitor an ASF infection. • We observe changes in two protein antibodies to dynamically monitor ASF infection. • We use diversified detection on a single test strip to detect both antibodies.
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