ArticleJournal of mass spectrometry and advances in the clinical lab2021
Endocannabinoids as potential biomarkers: It's all about pre-analytics.
Article in Journal of mass spectrometry and advances in the clinical lab, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Advances in targeted liquid chromatography-tandem mass spectrometry methods for endocannabinoid and N-acylethanolamine quantification in biological matrices: A systematic review.Mass spectrometry reviewsPooled it
- Longitudinal changes in endocannabinoids and clinical course of adolescent non-suicidal self-injury.Translational psychiatry · 2026Article
- Advanced analytical approaches for endocannabinoids and endocannabinoid-like substances: Applications in liver disease research.Journal of pharmaceutical analysis · 2026Review
- Palmitoylethanolamide in Human and Animal Obesity.Molecules (Basel, Switzerland) · 2026Review
- A Minimally Invasive LC-MS/MS Approach for Assessing Endocannabinoids in Saliva and Capillary Blood Microsamples.Biosensors · 2026Article
- Direct modulation of human GABA-A αFrontiers in neuroscience · 2026Review
- The Endocannabinoid-Microbiota-Neuroimmune Super-System: A Unifying Feedback Architecture for Systems Resilience, Collapse Trajectories, and Precision Feedback Medicine.International journal of molecular sciences · 2025Review
- Association of Serum Endocannabinoid Levels with Pancreatitis and Pancreatitis-Related Pain.Cannabis and cannabinoid research · 2025Article
- Alterations of the endocannabinoid system in adolescents with non-suicidal self-injury as a function of childhood maltreatment.Translational psychiatry · 2024Article
- The circulating and central endocannabinoid system in obesity and weight loss.International journal of obesity (2005) · 2024Review
- A Sensitive Ultrahigh-Performance Liquid Chromatography/Tandem Mass Spectrometry Method for the Simultaneous Analysis of Phytocannabinoids and Endocannabinoids in Plasma and Brain.Cannabis and cannabinoid research · 2024Article
- Genetic variation of the 5-HT1A rs6295, 5-HT2A rs6311, and CNR1 rs1049353 and an altered endocannabinoid system in depressed patients.Brain and behavior · 2023Article
- SPME as a green sample-preparation technique for the monitoring of phytocannabinoids and endocannabinoids in complex matrices.Journal of pharmaceutical analysis · 2023Review
- Monitoring of age- and gender-related alterations of endocannabinoid levels in selected brain regions with the use of SPME probes.Metabolomics : Official journal of the Metabolomic Society · 2023Article
- Pre-analytical sample handling standardization for reliable measurement of metabolites and lipids in LC-MS-based clinical research.Journal of mass spectrometry and advances in the clinical lab · 2023Article
- A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination.Nature communications · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionArachidonoyl ethanolamide (AEA) and 2-arachidonoyl glycerol (2-AG) are central lipid mediators of the endocannabinoid system. They are highly relevant due to their involvement in a wide variety of inflammatory, metabolic or malign diseases. Further elucidation of their modes of action and use as biomarkers in an easily accessible matrix, like blood, is restricted by their susceptibility to deviations during blood sampling and physiological co-dependences, which results in high variability of reported concentrations in low ng/mL ranges.
objectivesThe objective of this review is the identification of critical parameters during the pre-analytical phase and proposal of minimum requirements for reliable determination of endocannabinoids (ECs) in blood samples.
methodsReported physiological processes influencing the EC concentrations were put into context with published pre-analytical research and stability data from bioanalytical method validation.
resultsThe cause for variability in EC concentrations is versatile. In part, they are caused by inter-individual factors like sex, metabolic status and/or diurnal changes. Nevertheless, enzymatic activity in freshly drawn blood samples is the main reason for changing concentrations of AEA and 2-AG, besides additional non-enzymatic isomerization of the latter.
conclusionBlood samples for EC analyses require immediate processing at low temperatures (>0 °C) to maintain sample integrity. Standardization of the respective blood tube or anti-coagulant, sampling time point, applied centrifugal force and complete processing time can further decrease variability caused by sample handling. Nevertheless, extensive characterization of study participants is needed to reduce distortion of clinical data caused by co-variables and facilitate research on the endocannabinoid system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.