Evidence map›Paper›PMID 34938499›Full record

ArticleEcology and evolution2021

Selection of a reference gene for studies on lipid-related aquatic adaptations of toothed whales (

Jayan D M Senevirathna, Ryo Yonezawa, Taiki Saka, Yoji Igarashi, Noriko Funasaka, Kazutoshi Yoshitake, Shigeharu Kinoshita, Shuichi Asakawa

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ecology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Trial
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Jayan D M SenevirathnaLaboratory of Aquatic Molecular Biology and Biotechnology Department of Aquatic Bioscience Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan.ORCID https://orcid.org/0000-0002-9800-1962
Ryo YonezawaLaboratory of Aquatic Molecular Biology and Biotechnology Department of Aquatic Bioscience Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan.
Taiki SakaLaboratory of Aquatic Molecular Biology and Biotechnology Department of Aquatic Bioscience Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan.
Yoji IgarashiDepartment of Life Sciences and Chemistry Graduate School of Bioresources Mie University Mie Japan.
Noriko FunasakaDepartment of Life Sciences Graduate School of Bioresources Mie University Mie Japan.
Kazutoshi YoshitakeLaboratory of Aquatic Molecular Biology and Biotechnology Department of Aquatic Bioscience Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan.
Shigeharu KinoshitaLaboratory of Aquatic Molecular Biology and Biotechnology Department of Aquatic Bioscience Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan.
Shuichi AsakawaLaboratory of Aquatic Molecular Biology and Biotechnology Department of Aquatic Bioscience Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo Japan.
Tokyo University of Agriculture · JPMie University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Toothed whales are one group of marine mammals that has developed special adaptations, such as echolocation for predation, to successfully live in a dynamic aquatic environment. Their fat metabolism may differ from that of other mammals because toothed whales have acoustic fats. Gene expression in the metabolic pathways of animals can change with respect to their evolution and environment. A real-time quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for studying the relative expressions of genes. However, since the accuracy of RT-qPCR data is totally dependent on the reference gene, the selection of the reference gene is an essential step. In this study, 10 candidate reference genes (

Indexed as

cetaceangene expressiongene stabilityqPCRreference genetranscriptomics

Identifiers

PMID34938499
PMCPMC8668803
OpenAlexW3215260967

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.