ArticleBiotechnology for biofuels2021
Engineering Rhodosporidium toruloides for limonene production.
Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- Enhancing delta-tocotrienol production inSynthetic and systems biotechnology · 2026Article
- Biosynthesis of patchoulol via metabolic engineering the oleaginous red yeastSynthetic and systems biotechnology · 2026Article
- Bioactivities and biosynthesis of monoterpene-based biopesticides: current state and perspectives.Microbial cell factories · 2026Review
- Evaluating isoprenol production using the IPP-bypass pathway in the oleaginous yeast Rhodosporidium toruloides.Biotechnology for biofuels and bioproducts · 2026Article
- Introduction of human mMicrobial cell factories · 2025Article
- Biosynthesis of Edible Terpenoids: Hosts and Applications.Foods (Basel, Switzerland) · 2025Review
- Recent advances in genetic engineering and chemical production in yeast species.FEMS yeast research · 2025Review
- Efficient synthesis of limonene production in Yarrowia lipolytica by combinatorial engineering strategies.Biotechnology for biofuels and bioproducts · 2024Article
- Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.Molecules (Basel, Switzerland) · 2024Review
- Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.Bioresources and bioprocessing · 2023Article
- Modularly engineeringFrontiers in bioengineering and biotechnology · 2023Article
- Metabolic engineering of Rhodotorula toruloides for resveratrol production.Microbial cell factories · 2022Article
- Metabolic engineering of oleaginous yeast Rhodotorula toruloides for overproduction of triacetic acid lactone.Biotechnology and bioengineering · 2022Article
- Metabolic engineering of Ashbya gossypii for limonene production from xylose.Biotechnology for biofuels and bioproducts · 2022Article
- Translating advances in microbial bioproduction to sustainable biotechnology.Frontiers in bioengineering and biotechnology · 2022Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundLimonene is a widely used monoterpene in the production of food, pharmaceuticals, biofuels, etc. The objective of this work was to engineer Rhodosporidium toruloides as a cell factory for the production of limonene.
resultsBy overexpressing the limonene synthase (LS), neryl pyrophosphate synthase (NPPS)/geranyl pyrophosphate synthase and the native hydroxy-methyl-glutaryl-CoA reductase (HMGR), we established a baseline for limonene production based on the mevalonate route in Rhodosporidium toruloides. To further enhance the limonene titer, the acetoacetyl-CoA thiolase/HMGR (EfMvaE) and mevalonate synthase (EfMvaS) from Enterococcus faecalis, the mevalonate kinase from Methanosarcina mazei (MmMK) and the chimeric enzyme NPPS-LS were introduced in the carotenogenesis-deficient strain. The resulting strains produced a maximum limonene titer of 393.5 mg/L.
conclusionIn this study, we successfully engineered the carotenogenesis yeast R. toruloides to produce limonene. This is the first report on engineering R. toruloides toward limonene production based on NPP and the fusion protein SltNPPS-CltLS. The results demonstrated that R. toruloides is viable for limonene production, which would provide insights into microbial production of valuable monoterpenes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.