ArticleMicrobiology spectrum2021
Improved Formazan Dissolution for Bacterial MTT Assay.
Article in Microbiology spectrum, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
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- Whole genome surveying and metabolomic profiling aided navigation of therapeutic plethora of Streptomyces melanogenes WPF1 isolated from Pinus patula rhizosphere.Bioresources and bioprocessing · 2025Article
- Integrative network pharmacology and molecular dynamics analysis ofRSC advances · 2025Article
- Understanding interspecies drug response variations between human and rodent P2X7 receptors.Nature communications · 2025Article
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- A Dual Bioassay for Evaluation of Embryotoxicity and Acute Toxicity of Common Solvents and Surfactants inToxics · 2025Article
- Stevia Leaf Extract Fermented with Plant-DerivedInternational journal of molecular sciences · 2025Article
- A Novel Fluorescent Sensor for FeMolecules (Basel, Switzerland) · 2025Article
- A Pyrroloquinazoline Analogue Regulated Streptococcus mutans and Streptococcus sanguinis Dual-Species Biofilms.International dental journal · 2025Article
- Advances in gut microbiota functions in inflammatory bowel disease: Dysbiosis, management, cytotoxicity assessment, and therapeutic perspectives.Computational and structural biotechnology journal · 2025Review
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- Control of Postharvest Green Mold in Citrus by the Antimicrobial Peptide BP15 and Its Lipopeptides.Journal of fungi (Basel, Switzerland) · 2024Article
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- Mineral trioxide aggregate in membrane form as a barrier membrane in guided bone regeneration.Journal of dental sciences · 2024Article
- Development of a Convenient and Quantitative Method for Evaluating Photosensitizing Activity Using Thiazolyl Blue Formazan Dye.Molecules (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The MTT assay, based on the enzymatic reduction of the water-soluble, yellowish tetrazolium salt 3-(4,5-dimethylthiazol)-2,5-diphenyl-tetrazolium bromide (MTT) to purple formazan, is commonly used for assessment of cell viability and proliferation. Accurate performance by the MTT assay depends on complete solubilization of cells and formazan and stability of the colored solution. Comparison of different solubilization solutions revealed that dimethylformamide (DMF) and dimethyl sulfoxide (DMSO), buffered with ammonia buffer, pH 10, and containing 5% SDS, produced the best results. These two solvents provided rapid and complete solubilization of formazan and cells, with minimal background absorbance at 700 nm, good reproducibility (low interassay coefficient of variation), high sensitivity, and color stability for at least 24 h. A linear relationship between viable-cell number and formazan absorbance was preserved for cell densities up to ∼1 × 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.