ArticleCell reports2021
Virally encoded connectivity transgenic overlay RNA sequencing (VECTORseq) defines projection neurons involved in sensorimotor integration.
Article in Cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Efferent projections of topographically distinct relaxin family peptide receptor-3 (RXFP3) lateral hypothalamus/zona incerta cells.Brain structure & function · 2026Article
- CBLN2 promoter enables genetic access to wide-field neurons of the tree shrew superior colliculus.Cell reports methods · 2026Article
- Organization of mouse prefrontal cortex subnetwork revealed by spatial single-cell multi-omic analysis of SPIDER-Seq.National science review · 2026Article
- A Medial Paralemniscal Pth2ergic Circuit Contributing to Aversive and Anxiety-Like Behaviors.Neuroscience bulletin · 2026Article
- Accessing genetically defined cell types in the superior colliculus with transgenic mouse lines.iScience · 2025Article
- Single-nucleus profiling decoding the subcortical visual pathway evolution of vertebrates.iScience · 2025Article
- Genetically defined neuron types underlying visuomotor transformation in the superior colliculus.Nature reviews. Neuroscience · 2024Review
- High-throughput mapping of single-neuron projection and molecular features by retrograde barcoded labeling.eLife · 2024Article
- Single-nucleus RNA sequencing of developing superior colliculus identifies neuronal diversity and candidate mediators of circuit assembly.Cell reports · 2023Article
- Article
- Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries.Nature communications · 2022Article
- Transcriptional profiling of mouse projection neurons with VECTORseq.STAR protocols · 2022Article
- Advances in approaches to study cell-type specific cortical circuits throughout development.Frontiers in cellular neuroscience · 2022Review
- Cell-type specific transcriptomic signatures of neocortical circuit organization and their relevance to autism.Frontiers in neural circuits · 2022Article
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Authors and funding
6 authors.
Funding
Abstract
Behavior arises from concerted activity throughout the brain. Consequently, a major focus of modern neuroscience is defining the physiology and behavioral roles of projection neurons linking different brain areas. Single-cell RNA sequencing has facilitated these efforts by revealing molecular determinants of cellular physiology and markers that enable genetically targeted perturbations such as optogenetics, but existing methods for sequencing defined projection populations are low throughput, painstaking, and costly. We developed a straightforward, multiplexed approach, virally encoded connectivity transgenic overlay RNA sequencing (VECTORseq). VECTORseq repurposes commercial retrogradely infecting viruses typically used to express functional transgenes (e.g., recombinases and fluorescent proteins) by treating viral transgene mRNA as barcodes within single-cell datasets. VECTORseq is compatible with different viral families, resolves multiple populations with different projection targets in one sequencing run, and identifies cortical and subcortical excitatory and inhibitory projection populations. Our study provides a roadmap for high-throughput identification of neuronal subtypes based on connectivity.
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