ArticleBiomedica : revista del Instituto Nacional de Salud2021
Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes.
Article in Biomedica : revista del Instituto Nacional de Salud, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed, 4 citations in OpenAlex.
- Comparative Evaluation of Levamisole and Broccoli in Mitigating Testicular Oxidative Stress and Apoptotic Alterations Caused by Cadmium and Lead Exposure in Rats.Biological trace element research · 2025Article
- The Effectiveness of Time in Treatment with Vitamin C and Broccoli Extract on Cadmium Poisoning in Mice: Histological Changes of Testicular Tissue and Cell Apoptotic Index.Biological trace element research · 2024Article
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4 authors at 1 institution in 1 country.
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Abstract
introductionBroccoli (Brassica oleracea) is well known for its properties as an anticancer, antioxidant, and scavenger of free radicals. However, its benefits in enhancing spermatogenesis have not been well established.
objectiveTo study broccoli aqueous extract effects on sperm factors and the expression of genes Catsper1, Catsper2, Arl4a, Sox5, and Sox9 in sperm factors in mice. MATERIAL AND
methodsMale mice were divided randomly into six groups: (1) Control; (2) cadmium (3 mg/kg of mouse body weight); (3) orally treated with 200 μl broccoli aqueous extract (1 g ml-1); (4) orally treated with 400 μl of broccoli aqueous extract; (5) orally treated with 200 broccoli aqueous extract plus cadmium, and (6) orally treated with 400 μl of broccoli aqueous extract plus cadmium. We analyzed the sperms factors and Catsper1, Catsper2, Arl4a, Sox5, and Sox9 gene expression.
resultsAn obvious improvement in sperm count and a slight enhancement in sperm motility were observed in mice treated with broccoli extract alone or with cadmium. Sperm viability was reduced by broccoli extract except for the 200 μl dose with cadmium, which significantly increased it. Interestingly, Arl4a gene expression increased in the 400 μl broccoli-treated group. Likewise, the Arl4a mRNA level in mice treated with cadmium and 200 μl of broccoli extract was higher than in the cadmium-treated mice. Furthermore, broccoli extract enhanced the mRNA level of Catsper2 and Sox5 genes in mice treated with 200 μl and 400 μl broccoli extract plus cadmium compared with the group treated solely with cadmium.
conclusionThe higher sperm count in broccoli-treated mice opens the way for the development of pharmaceutical products for infertile men.
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