Evidence map›Paper›PMID 34935907›Full record

ArticleBiology open2021

Dynamic shapes of the zygote and two-cell mouse and human.

Chris F Graham, Shane Windsor, Anna Ajduk, Thanh Trinh, Anna Vincent, Celine Jones, Kevin Coward, Dilraj Kalsi, Magdalena Zernicka-Goetz, Karl Swann and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 3 countries.

Chris F GrahamZoology Department, University of Oxford, 11a Mansfield Road, Oxford, OX1 3SZ, UK.ORCID 0000-0002-6410-8171
Shane WindsorDepartment of Aerospace Engineering, University of Bristol, Queens Building, University Walk, Bristol, BS8 1TR, UK.ORCID 0000-0002-7597-4497
Anna AjdukDepartment of Embryology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, POLAND.ORCID 0000-0002-7262-1370
Thanh TrinhNuffield Department of Women's Reproductive Health, Level 3, Women's Centre, John Radcliffe Hospital, Oxford, OX3 9DU, UK.ORCID 0000-0002-6973-9749
Anna VincentOxford Fertility, Oxford University, Oxford Business Park North, Alec Issigonis Way, Oxford, OX4 2HW, UK.
Celine JonesNuffield Department of Women's Reproductive Health, Level 3, Women's Centre, John Radcliffe Hospital, Oxford, OX3 9DU, UK.
Kevin CowardNuffield Department of Women's Reproductive Health, Level 3, Women's Centre, John Radcliffe Hospital, Oxford, OX3 9DU, UK.
Dilraj KalsiNuffield Department of Surgical Sciences, University of Oxford, Oxford, OX3 9D, UK.ORCID 0000-0001-9300-1835
Magdalena Zernicka-GoetzDepartment of Physiology, Development and Neuroscience, Downing Street, Cambridge, CB23 3EL, UK.ORCID 0000-0002-7004-2471
Karl SwannSchool of Biosciences, Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, UK.ORCID 0000-0002-4355-1449
Adrian L R ThomasZoology Department, University of Oxford, 11a Mansfield Road, Oxford, OX1 3SZ, UK.ORCID 0000-0003-0878-4113
John Radcliffe Hospital · GBUniversity of Oxford · GBCardiff University · GBCleveland Clinic · USUniversity of Bristol · GBUniversity of Cambridge · GBUniversity of Warsaw · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mouse zygote morphokinetics were measured during interphase, the mitotic period, cytokinesis, and two-cell stage. Sequences of rounder-distorted-rounder shapes were revealed, as were changing patterns of cross section area. A calcium chelator and an actin-disrupting agent inhibited the area changes that occurred between pronuclear envelope breakdown and cytokinesis. During cell division, two vortices developed in each nascent cell and they rotated in opposite directions at each end of the cell, a pattern that sometimes persisted for up to 10 h. Exchange with the environment may have been promoted by these shape and area cycles and persisting circulation in the cytoplasm may have a similar function between a cell's interior and periphery. Some of these movements were sporadically also seen in human zygotes with abnormal numbers of pronuclei and the two-cell stages that developed from these compromised human zygotes.

Indexed as

Cell NucleusZygoteAnimalsCytoplasmHumansMiceCytoplasm vorticesHumanMorphokineticsMouse zygotePronuclear fadingShape cycles

Identifiers

PMID34935907
PMCPMC8713988
OpenAlexW4200034069

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.