Evidence map›Paper›PMID 34934095›Full record

ArticleScientific reports2021

circRNA N6-methyladenosine methylation in preeclampsia and the potential role of N6-methyladenosine-modified circPAPPA2 in trophoblast invasion.

Yonggang Zhang, Hongling Yang, Yan Long, Yipeng Zhang, Ronggui Chen, Junzhu Shi, Jiying Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 50 citations in OpenAlex.

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  14. Epigenetics and environmental health.Frontiers of medicine · 2024
    Review
  15. Article
  16. Current understanding of circular RNAs in preeclampsia.Hypertension research : official journal of the Japanese Society of Hypertension · 2024
    Review
  17. Article
  18. Article
  19. Article
  20. N6-methyladenosine (m6A) Modification in Preeclampsia.Reproductive sciences (Thousand Oaks, Calif.) · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yonggang ZhangDepartment of Clinical Laboratory, Shenzhen Longhua District Central Hospital, Guangdong Medical University, No. 187 Guanlan Avenue, Shenzhen, 518110, Guangdong, China. ygzhang62@sina.com.
Hongling YangDepartment of Clinical Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, Guangdong, China.
Yan LongDepartment of Clinical Laboratory, Guangzhou Women and Children's Medical Centre, Guangzhou Medical University, Guangzhou, 510623, Guangdong, China.
Yipeng ZhangDepartment of Clinical Laboratory, Shenzhen Longhua District Central Hospital, Guangdong Medical University, No. 187 Guanlan Avenue, Shenzhen, 518110, Guangdong, China.
Ronggui ChenDepartment of Clinical Laboratory, Shenzhen Longhua District Central Hospital, Guangdong Medical University, No. 187 Guanlan Avenue, Shenzhen, 518110, Guangdong, China. 13500053521@163.com.
Junzhu ShiDepartment of Clinical Laboratory, Shenzhen Longhua District Central Hospital, Guangdong Medical University, No. 187 Guanlan Avenue, Shenzhen, 518110, Guangdong, China.
Jiying ChenDepartment of Obstetrics and Gynecology, Shenzhen Longhua District Central Hospital, Guangdong Medical University, Shenzhen, 518110, Guangdong, China.
Guangdong Medical College · CNGuangzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we performed N6-methyladenosine (m6A) RNA sequencing to determine the circRNA m6A methylation changes in the placentas during the pathogenesis of preeclampsia (PE). We verified the expression of the circRNA circPAPPA2 using quantitative reverse transcription-PCR. An invasion assay was carried out to identify the role of circPAPPA2 in the development of PE. Mechanistically, we investigated the cause of the altered m6A modification of circPAPPA2 through overexpression and knockdown cell experiments, RNA immunoprecipitation, fluorescence in situ hybridization and RNA stability experiments. We found that increases in m6A-modified circRNAs are prevalent in PE placentas and that the main changes in methylation occur in the 3'UTR and near the start codon, implicating the involvement of these changes in PE development. We also found that the levels of circPAPPA2 are decreased but that m6A modification is augmented. Furthermore, we discovered that methyltransferase‑like 14 (METTL14) increases the level of circPAPPA2 m6A methylation and that insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) maintains circPAPPA2 stability. Decreases in IGF2BP3 levels lead to declines in circPAPPA2 levels. In summary, we provide a new vision and strategy for the study of PE pathology and report that placental circRNA m6A modification appears to be an important regulatory mechanism.

Indexed as

Gene Expression RegulationAdenosineCase-Control StudiesFemaleHumansMethyltransferasesPlacentaPre-EclampsiaPregnancyPregnancy-Associated Plasma Protein-ARNA-Binding ProteinsRNA, CircularTrophoblastsAdenosineIGF2BP3 protein, humanMethyltransferasesMETTL14 protein, humanN-methyladenosinePAPPA2 protein, humanPregnancy-Associated Plasma Protein-ARNA-Binding ProteinsRNA, Circular

Identifiers

PMID34934095
PMCPMC8692596
OpenAlexW4200185170

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.