Evidence map›Paper›PMID 34932571›Full record

ArticlePloS one2021

Identification of starch candidate genes using SLAF-seq and BSA strategies and development of related SNP-CAPS markers in tetraploid potato.

Jiaqi Li, Xiaoxia Yu, Sheng Zhang, Zhuo Yu, Jingwei Li, Xinghong Jin, Xia Zhang, Dongsheng Yang

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Jiaqi LiAgricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Xiaoxia YuAgricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Sheng ZhangAgricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.ORCID 0000-0003-0656-8206
Zhuo YuAgricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Jingwei LiAgricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Xinghong JinAgricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Xia ZhangAgricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Dongsheng YangAgricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Inner Mongolia Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Potato starch is an essential nutrient for humans and is widely used worldwide. Locating relevant genomic regions, mining stable genes and developing candidate gene markers can promote the breeding of new high-starch potato varieties. A total of 106 F1 individuals and their parents (YSP-4 × MIN-021) were used as test materials, from which 20 plants with high starch content and 20 with low starch content were selected to construct DNA pools for site-specific amplified fragment sequencing (SLAF-seq) and bulked segregation analysis (BSA). A genomic region related to the starch traits was first identified in the 0-5.62 Mb of chromosome 2 in tetraploid potato. In this section, a total of 41 non-synonymous genes, which were considered as candidate genes related to the starch trait, were annotated through a basic local alignment search tool (BLAST) search of multiple databases. Six candidate genes for starch (PGSC0003DMG400017793, PGSC0003DMG400035245, PGSC0003DMG400036713, PGSC0003DMG400040452, PGSC0003DMG400006636 and PGSC0003DMG400044547) were further explored. In addition, cleaved amplified polymorphic sequence (CAPS) markers were developed based on single nucleotide polymorphism (SNP) sites associated with the starch candidate genes. SNP-CAPS markers chr2-CAPS6 and chr2-CAPS21 were successfully developed and validated with the F2 population and 24 tetraploid potato varieties (lines). Functional analysis and cloning of the candidate genes associated with potato starch will be performed in further research, and the SNP-CAPS markers chr2-CAPS6 and chr2-CAPS21 can be further used in marker-assisted selection breeding of tetraploid potato varieties with high starch content.

Indexed as

Gene Expression Regulation, PlantPolymorphism, Single NucleotideTetraploidyBiomarkersChromosomes, PlantPhenotypePlant ProteinsQuantitative Trait LociSolanum tuberosumStarchBiomarkersPlant ProteinsStarch

Identifiers

PMID34932571
PMCPMC8691606
OpenAlexW4200311548

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.