ArticleeLife2021
3D virtual histopathology of cardiac tissue from Covid-19 patients based on phase-contrast X-ray tomography.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- An Optimized Freeze-Dry Multimodal Workflow for Sequential Micro-CT Imaging, Histology, and Molecular Profiling: A Use Case in Human Liver Fibrosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Bridging the microstructural gap in human connectomics using hierarchical phase-contrast tomography as a reference for diffusion MRI in the human brain.bioRxiv : the preprint server for biology · 2026Article
- Quantitative Stain Mapping in X-Ray Virtual Histology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Three-dimensional high-content imaging of unstained soft tissue with subcellular resolution using a laboratory-based X-ray microscope.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Whole-heart 3D reconstruction of mouse CVB3 myocarditis reveals spatial and transcriptomic heterogeneity of immune foci.Cardiovascular research · 2025Article
- 3D histology of human heart-forming organoids by X-ray phase-contrast tomography.Communications biology · 2025Article
- Intussusceptive angiogenesis-on-a-chip: Evidence for transluminal vascular bridging by endothelial delamination.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Three-dimensional structure of entire hydrated murine hearts at histological resolution.Scientific reports · 2025Article
- 3D multiscale characterization of the human placenta: Bridging anatomy and histology by X-ray phase-contrast tomography.PNAS nexus · 2025Article
- Cryo-X-Ray Phase Contrast Imaging Enables Combined 3D Structural Quantification and Nucleic Acid Analysis of Myocardial Biopsies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Deep learning for 3D vascular segmentation in hierarchical phase contrast tomography: a case study on kidney.Scientific reports · 2024Article
- Synchrotron X-ray imaging of soft biological tissues - principles, applications and future prospects.Journal of cell science · 2024Review
- Deep Learning for 3D Vascular Segmentation in Phase Contrast Tomography.Research square · 2024Article
- 3D imaging of SARS-CoV-2 infected hamster lungs by X-ray phase contrast tomography enables drug testing.Scientific reports · 2024Article
- Article
- Evaluation of MR-safe bioptomes for MR-guided endomyocardial biopsy in minipigs: a potential radiation-free clinical approach.European radiology experimental · 2023Article
- Neodymium acetate as a contrast agent for X-ray phase-contrast tomography.Journal of medical imaging (Bellingham, Wash.) · 2023Article
- Three-dimensional analysis of human pancreatic cancer specimens by phase-contrast based X-ray tomography - the next dimension of diagnosis.Cancer imaging : the official publication of the International Cancer Imaging Society · 2023Article
- Deep Survival Analysis With Clinical Variables for COVID-19.IEEE journal of translational engineering in health and medicine · 2023Article
- [On the way to the virtual microscope: synchrotron-radiation-based three-dimensional imaging in pathology].Pathologie (Heidelberg, Germany) · 2022Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
For the first time, we have used phase-contrast X-ray tomography to characterize the three-dimensional (3d) structure of cardiac tissue from patients who succumbed to Covid-19. By extending conventional histopathological examination by a third dimension, the delicate pathological changes of the vascular system of severe Covid-19 progressions can be analyzed, fully quantified and compared to other types of viral myocarditis and controls. To this end, cardiac samples with a cross-section of 3.5mm were scanned at a laboratory setup as well as at a parallel beam setup at a synchrotron radiation facility the synchrotron in a parallel beam configuration. The vascular network was segmented by a deep learning architecture suitable for 3d datasets (V-net), trained by sparse manual annotations. Pathological alterations of vessels, concerning the variation of diameters and the amount of small holes, were observed, indicative of elevated occurrence of intussusceptive angiogenesis, also confirmed by high-resolution cone beam X-ray tomography and scanning electron microscopy. Furthermore, we implemented a fully automated analysis of the tissue structure in the form of shape measures based on the structure tensor. The corresponding distributions show that the histopathology of Covid-19 differs from both influenza and typical coxsackie virus myocarditis.
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