ArticlePloS one2021
Selection and validation of reference genes for normalization of qRT-PCR data to study the cannabinoid pathway genes in industrial hemp.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 14 citations in OpenAlex.
- An Integrated Transient Expression and UPLC-PDA Approach for Functional Genomics and Metabolic Analysis of Cannabis sativa.Biotechnology journal · 2026Article
- Long non-coding RNA landscape and ceRNA networks underlying the drought response of Cannabis sativa L.BMC plant biology · 2026Article
- Expression and Promoter Methylation of the Genes Encoding the Mitochondrial and Cytosolic Forms of Fumarase in Sunflower (Current issues in molecular biology · 2026Article
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
- Transcriptomic analysis of CDL-gated photoperiodic flowering mechanisms in cannabis and their responsiveness to R: FR ratios in controlled environment agriculture.Scientific reports · 2025Article
- Effect of Hormonal Treatments on Cannabinoid Content Levels in Female Hemp (International journal of molecular sciences · 2025Article
- Phenotypic and genetic characterization of sixteen grain and dual-type industrial hemp varieties (Frontiers in plant science · 2025Article
- Early protoplast culture and partial regeneration inFrontiers in plant science · 2025Article
- CRISP: A cost-efficient, high-throughput pipeline for sex-identification inmicroPublication biology · 2025Article
- Transcriptome-guided selection of stable reference genes for expression analysis in spinach.Scientific reports · 2024Article
- Sensing Biomolecules Associated with Cells' Radiosusceptibility by Advanced Micro- and Nanospectroscopy Techniques.ACS sensors · 2024Article
- Article
- Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments inInternational journal of molecular sciences · 2023Article
- Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There has been significant interest in researching the pharmaceutical applications of Industrial hemp since its legalization three years ago. The crop is mostly dioecious and known for its production of phytocannabinoids, flavonoids, and terpenes. Although many scientific reports have showed gene expression analysis of hemp through OMICs approaches, unreliable reference genes for normalization of qRT-PCR data make it difficult to validate the OMICs data. Four software packages: geNorm, NormFinder, BestKeeper, and RefFinder were used to evaluate the differential gene expression patterns of 13 candidate reference genes under osmotic, heavy metal, hormonal, and UV stresses. EF-1α ranked as the most stable reference gene across all stresses, TUB was the most stable under osmotic stress, and TATA was the most stable under both heavy metal stress and hormonal stimuli. The expression patterns of two cannabinoid pathway genes, AAE1 and CBDAS, were used to validate the reliability of the selected reference genes. This work provides useful information for gene expression characterization in hemp and future research in the synthesis, transport, and accumulation of secondary metabolites.
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