ArticleMolecular systems biology2021
Deciphering the physiological response of Escherichia coli under high ATP demand.
Article in Molecular systems biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Sodium ion stress accelerates cell lysis inApplied and environmental microbiology · 2026Article
- Genetically encoded manipulation of ATP/ADP ratio in human cells uncovers proteomic and physiological signatures of energy stress.Cell chemical biology · 2026Article
- Coordinating Calvin Cycle and Glycolysis inACS synthetic biology · 2026Article
- Precision Proteolysis of Triosephosphate Isomerase ofACS synthetic biology · 2026Article
- COBRA-k: A powerful framework bridging constraint-based and kinetic metabolic modeling.Science advances · 2026Article
- Genetic make-up and regulation of the L-lysine biosynthesis pathway inMicrobial cell (Graz, Austria) · 2026Article
- Quantitative Coarse-Grained Modeling of Microbial Metabolism: Uncovering Growth Laws, Environmental Responses, and Host-Circuit Coupling.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Nitroxoline as an antimicrobial synergist reverses colistin resistance in multidrug-resistantiScience · 2025Article
- Glycolytic ATP production enables rapid mammalian cell growth.bioRxiv : the preprint server for biology · 2025Article
- Bioenergetic stress potentiates antimicrobial resistance and persistence.Nature communications · 2025Article
- Mer overexpression inApplied and environmental microbiology · 2025Article
- Article
- Biotechnology systems engineering: preparing the next generation of bioengineers.Frontiers in systems biology · 2025Article
- Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli.NPJ systems biology and applications · 2024Article
- Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.Nucleic acids research · 2024Article
- Engineering new-to-nature biochemical conversions by combining fermentative metabolism with respiratory modules.Nature communications · 2024Article
- ATP biosensor reveals microbial energetic dynamics and facilitates bioproduction.Nature communications · 2024Article
- Transcriptional and Metabolic Response of a Strain ofBiotech (Basel (Switzerland)) · 2024Article
- Balancing biomass reaction stoichiometry and measured fluxes in flux balance analysis.Bioinformatics (Oxford, England) · 2023Article
- Growth-coupled anaerobic production of isobutanol from glucose in minimal medium with Escherichia coli.Biotechnology for biofuels and bioproducts · 2023Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One long-standing question in microbiology is how microbes buffer perturbations in energy metabolism. In this study, we systematically analyzed the impact of different levels of ATP demand in Escherichia coli under various conditions (aerobic and anaerobic, with and without cell growth). One key finding is that, under all conditions tested, the glucose uptake increases with rising ATP demand, but only to a critical level beyond which it drops markedly, even below wild-type levels. Focusing on anaerobic growth and using metabolomics and proteomics data in combination with a kinetic model, we show that this biphasic behavior is induced by the dual dependency of the phosphofructokinase on ATP (substrate) and ADP (allosteric activator). This mechanism buffers increased ATP demands by a higher glycolytic flux but, as shown herein, it collapses under very low ATP concentrations. Model analysis also revealed two major rate-controlling steps in the glycolysis under high ATP demand, which could be confirmed experimentally. Our results provide new insights on fundamental mechanisms of bacterial energy metabolism and guide the rational engineering of highly productive cell factories.
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