ArticleFrontiers in molecular biosciences2021
Simple and Feasible Detection of Hepatitis B Virus
Article in Frontiers in molecular biosciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Development of a MIRA-CRISPR/Cas12a-based nucleic acid detection system for the discrimination ofJournal of ginseng research · 2026Article
- Rapid Detection of Peste Des Petits Ruminants via Multienzyme Isothermal and Lateral Flow Dipstick Combination Assay Based on N Gene.Veterinary sciences · 2026Article
- Detection ofMicroorganisms · 2025Article
- Evaluation and Application of the MIRA-qPCR Method for Rapid Detection of Norovirus Genogroup II in Shellfish.Microorganisms · 2025Article
- Visible and rapid detection of feline chaphamaparvovirus using multienzyme isothermal rapid amplification and lateral flow dipstick assay.Frontiers in cellular and infection microbiology · 2025Article
- Rapid and sensitive detection of chikungunya virus using one-tube, reverse transcription, semi-nested multi-enzyme isothermal rapid amplification, and lateral flow dipstick assays.Journal of clinical microbiology · 2024Article
- Simple and field-adapted species identification of biological specimens combining multiplex multienzyme isothermal rapid amplification, lateral flow dipsticks, and universal primers for initial rapid screening without standard PCR laboratory.International journal of legal medicine · 2024Article
- A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalis.Parasites & vectors · 2024Article
- Rapid detection of human cytomegalovirus by multienzyme isothermal rapid amplification and lateral flow dipsticks.Frontiers in cellular and infection microbiology · 2024Article
- Development of a multienzyme isothermal and lateral flow dipstick combination assay for the rapid detection of goose astrovirus II.Frontiers in cellular and infection microbiology · 2024Article
- Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples.AMB Express · 2023Article
- Review
- Lateral Flow Assay for Hepatitis B Detection: A Review of Current and New Assays.Micromachines · 2023Review
- Development and Evaluation of Duplex MIRA-qPCR Assay for Simultaneous Detection of Staphylococcus aureus and non-aureus StaphylococciMicroorganisms · 2022Article
- Rapid detection ofFrontiers in microbiology · 2022Article
- Development and evaluation of a rapid and sensitive multienzyme isothermal rapid amplification with a lateral flow dipstick assay for detection ofFrontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatitis B virus infection is not only a huge burden in the field of social health but also a major public health problem that affects the lives and health of the people. Simple, rapid, feasible detection of HBV is critical for its prevention and spread, especially in the developing countries with low-resource laboratories. To this end, we combined multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) strip to detect HBV. A pair of primers targeting the conserved region of HBV genome was designed and used in MIRA-LFD assay. Our results found that the entire amplification of MIRA-LFD only takes 10 min at 37°C and the dilution of the amplification products was added in the LFD strip and observed by the naked eye after 10 min. The detection sensitivity of this method can reach 10 pg. The 45 clinical samples were detected by MIRA-LFD and real-time PCR. The accuracy rate of MIRA-LFD was 100%. Therefore, these characteristics of our newly developed MIRA-LFD assay make it particularly useful and suitable for detecting HBV in the resource-limited condition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.