ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2021
Screening of stable internal reference gene of Quinoa under hormone treatment and abiotic stress.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Comprehensive transcriptomic profiling identifies key regulatory genes mediating phytohormone signaling pathways during seed germination in Chenopodium quinoa.BMC genomics · 2026Article
- QuinoaFrontiers in plant science · 2026Article
- Screening of qPCR Reference Genes in Quinoa Under Cold, Heat, and Drought Gradient Stress.Plants (Basel, Switzerland) · 2025Article
- qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Current issues in molecular biology · 2025Article
- Identification of two new GRAS transcription factors and expression analysis of these genes inFrontiers in plant science · 2025Article
- Fungal endophytes boost salt tolerance and seed quality in quinoa ecotypes along a latitudinal gradient.Frontiers in plant science · 2025Article
- Comparative transcriptomes and WGCNA reveal hub genes for spike germination in different quinoa lines.BMC genomics · 2024Article
- Identification and characterization of the Quinoa AP2/ERF gene family and their expression patterns in response to salt stress.Scientific reports · 2024Article
- Identification and validation of qRT-PCR reference genes for analyzing grape infection with gray mold.BMC genomics · 2024Article
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- Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments inInternational journal of molecular sciences · 2023Article
- Screening and Validation of Appropriate Reference Genes for Real-Time Quantitative PCR under PEG, NaCl and ZnSOInternational journal of molecular sciences · 2023Article
- Selection of Reference Genes inPlants (Basel, Switzerland) · 2023Article
- Article
- Selection and verification of reliable internal reference genes in stem development of herbaceous peony (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Validation of Appropriate Reference Genes for qRT-PCR Normalization in Oat (International journal of molecular sciences · 2022Article
- Identification and Validation of Common Reference Genes for Normalization of Esophageal Squamous Cell Carcinoma Gene Expression Profiles.BioMed research international · 2022Article
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4 authors.
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Abstract
Real-time quantitative polymerase chain reaction is the most commonly used method to accurately detect gene expression patterns. The method requires stable internal reference genes to standardize the data. However, studies have shown that there is no stable expression of internal reference genes in different tissues and under different treatments. Therefore, in order to study the optimal reference genes of quinoa under different hormones and abiotic stress, leaves and stems from quinoa seedlings treated with low temperature (4 °C), salt (200 mmol/L) and abscisic acid (200 mmol/L) were used as experimental materials. Using ACT-1, eIF, EF1α, GAPDH, TUA, TUB-9, TUB-1, H2A and L8-1 as candidate reference genes, the expression stability of these 9 quinoa candidate reference genes under different hormone treatment and abiotic stress was evaluated by using geNorm, NormFinder and BestKeeper software. The results showed that TUB-1 gene under salt stress, L8-1 gene under low temperature stress, EF-1α gene induced by ABA. PLIM2c WLIM1and WLIM2b were selected to verify the candidate internal reference genes, and finally the expression of GAPDH was most unstable under the three treatments, which was not suitable to be the internal reference gene of quinoa under specific conditions, while EF1α showed good stability under the three different treatments and was suitable to be used as the internal reference gene. In conclusion, the results of this study could provide an important reference for quantifying the expression level of reference genes in quinoa. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-021-01094-z.
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