ArticleJournal of Alzheimer's disease : JAD2022
Effect of Long-Term Storage on the Reliability of Blood Biomarkers for Alzheimer's Disease and Neurodegeneration.
Article in Journal of Alzheimer's disease : JAD, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 28 citations in OpenAlex.
- Plasma Biomarkers for Alzheimer's Disease Across the Continuum: A Systematic Review of SIMOA-Based Studies.Cellular and molecular neurobiology · 2026Pooled it
- Neurofilament Light Chains in Systemic Amyloidosis: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Association of inflammation with glycemic control, insulin sensitivity, and beta-cell function in diabetic cats.Journal of veterinary internal medicine · 2026Article
- Midlife sensory and motor measures among best predictors in parsimonious models of long-term cognitive decline and incidence of cognitive impairment in aging adults.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026Article
- Increased Blood Levels of NfL, GFAP, and Placental Growth Factor After Radiotherapy to the Brain.Annals of clinical and translational neurology · 2026Article
- Ethical Biobanking in Extremely Preterm Infants: Lessons From the EPITOP Study.Acta paediatrica (Oslo, Norway : 1992) · 2025Article
- Sex differences on tau, astrocytic, and neurodegenerative plasma biomarkers.Journal of Alzheimer's disease : JAD · 2025Article
- The impact of pre-analytical factors on plasma biomarkers for Alzheimer's disease: The ASPREE Healthy Ageing Biobank.The journal of prevention of Alzheimer's disease · 2025Article
- A turn-on fluorescent immunosensor for neurodegenerative disease related neurofilament light chain protein.Mikrochimica acta · 2025Article
- Plasma neurofilament light chain as prognostic marker of cognitive decline in neurodegenerative diseases, a clinical setting study.Alzheimer's research & therapy · 2024Article
- Matching proposed clinical and MRI criteria of aggressive multiple sclerosis to serum and cerebrospinal fluid markers of neuroaxonal and glial injury.Journal of neurology · 2024Article
- Plasma Markers of Alzheimer's Disease Pathology, Neuronal Injury, and Astrocytic Activation and MRI Load of Vascular Pathology and Neurodegeneration: The SMART-MR Study.Journal of the American Heart Association · 2024Article
- Presence of Preoperative Neurodegeneration Biofluid Markers in Patients with Postoperative Delirium.Anesthesiology · 2023Article
- Cognitive Impairment in Multiple Sclerosis.Bioengineering (Basel, Switzerland) · 2023Review
- Neurofilament-light chain quantification by Simoa and Ella in plasma from patients with dementia: a comparative study.Scientific reports · 2023Article
- The impact of aging and oxidative stress in metabolic and nervous system disorders: programmed cell death and molecular signal transduction crosstalk.Frontiers in immunology · 2023Review
- Associations of Midlife Lifestyle and Health Factors with Long-Term Changes in Blood-Based Biomarkers of Alzheimer's Disease and Neurodegeneration.Journal of Alzheimer's disease : JAD · 2023Article
- Associations of sensory and motor function with blood-based biomarkers of neurodegeneration and Alzheimer's disease in midlife.Neurobiology of aging · 2022Article
- Midlife sensory and motor functions improve prediction of blood-based measures of neurodegeneration and Alzheimer's disease in late middle-age.Alzheimer's & dementia (Amsterdam, Netherlands)Article
- Use of neurofilament light chain to identify structural brain diseases in dogs.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundStored blood samples from longitudinal cohort studies may be useful for studying biomarkers of preclinical Alzheimer's disease.
objectiveThis study aimed to determine the reliability of amyloid-β40 and amyloid-β42 (Aβ40, Aβ42), total tau (TTau), and neurofilament light (NfL) concentrations measured in blood samples stored long-term at -80°C.
methodsAβ40, Aβ42, TTau, and NfL were measured in serum and plasma samples from two longitudinal cohort studies. Serum samples had been stored at -80°C for 5 (n = 24), 14 (n = 24), and 20 years (N = 78) and plasma samples had been stored for 16 years (N = 78). Biomarker concentrations were measured in duplicate using a single molecule array assay (Simoa; Quanterix, Billerica, MA). Replicate samples for each sample type and storage length were included.
resultsThe concentrations of Aβ40, Aβ42, TTau, and NfL were within expected ranges. Some serum TTau concentrations were below the limit of detection. The average intra-assay coefficients of variation (CV) for duplicate measures were 2-7% for all assays except for serum TTau, which were higher (CVs 13% and 17%). Mean differences in original replicate pair Aβ40, Aβ42, and NfL concentrations were slightly greater in samples stored for longer versus shorter time periods.
conclusionAβ40, Aβ42, TTau, and NfL can be measured in serum and plasma samples that have been stored up to 20 years at -80°C. Long-term storage may be associated with small increases in the variability of concentrations in samples stored 14 or more years.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.