Evidence map›Paper›PMID 34923968›Full record

ArticleJournal of biomedical science2021

The overexpression of GDNF in nucleus accumbens suppresses alcohol-seeking behavior in group-housed C57Bl/6J female mice.

Maryna Koskela, T Petteri Piepponen, Maria Lindahl, Brandon K Harvey, Jaan-Olle Andressoo, Vootele Võikar, Mikko Airavaara

Open access · diamondAbstract read
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In one paragraph

Article in Journal of biomedical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 4 countries.

Maryna KoskelaInstitute of Biotechnology, HiLIFE, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland.
T Petteri PiepponenDivision of Pharmacology and Pharmacotherapy, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland.
Maria LindahlInstitute of Biotechnology, HiLIFE, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland.
Brandon K HarveyNational Institute on Drug Abuse, IRP, NIH, Biomedical Research Center, 251 Bayview Boulevard Suite 200, Baltimore, MD, 21224, USA.
Jaan-Olle AndressooFaculty of Medicine, University of Helsinki, PO Box 56, 00014, Helsinki, Finland.
Vootele VõikarNeuroscience Center, HiLIFE, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland.
Mikko AiravaaraDivision of Pharmacology and Pharmacotherapy, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland. mikko.airavaara@helsinki.fi.ORCID http://orcid.org/0000-0002-2026-1609
University of Helsinki · FINational Institute on Drug Abuse · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCraving for alcohol, in other words powerful desire to drink after withdrawal, is an important contributor to the development and maintenance of alcoholism. Here, we studied the role of GDNF (glial cell line-derived neurotrophic factor) and BDNF (brain-derived neurotrophic factor) on alcohol-seeking behavior in group-housed female mice.

methodsWe modeled alcohol-seeking behavior in C57Bl/6J female mice. The behavioral experiments in group-housed female mice were performed in an automated IntelliCage system. We conducted RT-qPCR analysis of Gdnf, Bdnf, Manf and Cdnf expression in different areas of the female mouse brain after alcohol drinking conditioning. We injected an adeno-associated virus (AAV) vector expressing human GDNF or BDNF in mouse nucleus accumbens (NAc) after ten days of alcohol drinking conditioning and assessed alcohol-seeking behavior. Behavioral data were analyzed by two-way repeated-measures ANOVA, and statistically significant effects were followed by Bonferroni's post hoc test. The student's t-test was used to analyze qPCR data.

resultsThe RT-qPCR data showed that Gdnf mRNA level in NAc was more than four times higher (p < 0.0001) in the mice from the sweetened alcohol group compared to the water group. Our data showed a more than a two-fold decrease in Manf mRNA (p = 0.04) and Cdnf mRNA (p = 0.02) levels in the hippocampus and Manf mRNA in the VTA (p = 0.04) after alcohol consumption. Two-fold endogenous overexpression of Gdnf mRNA and lack of CDNF did not affect alcohol-seeking behavior. The AVV-GDNF overexpression in nucleus accumbens suppressed alcohol-seeking behavior while overexpression of BDNF did not.

conclusionsThe effect of increased endogenous Gdnf mRNA level in female mice upon alcohol drinking has remained unknown. Our data suggest that an increase in endogenous GDNF expression upon alcohol drinking occurs in response to the activation of another mesolimbic reward pathway participant.

Indexed as

CravingGene Expression RegulationAlcohol DrinkingAnimalsFemaleGlial Cell Line-Derived Neurotrophic FactorMiceMice, Inbred C57BLNucleus AccumbensSocial BehaviorGdnf protein, mouseGlial Cell Line-Derived Neurotrophic FactorAlcohol addictionConditional stimuliCravingIntelliCageSocial interaction, GDNF, BDNF

Identifiers

PMID34923968
PMCPMC8686589
OpenAlexW4200481924

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.