Evidence map›Paper›PMID 34923569›Full record

ArticleLaboratory investigation; a journal of technical methods and pathology2022

SIRT6 regulates SREBP1c-induced glucolipid metabolism in liver and pancreas via the AMPKα-mTORC1 pathway.

Che Bian, Haibo Zhang, Jing Gao, Yuxia Wang, Jia Li, Dan Guo, Wei Wang, Yuling Song, Yang Weng, Huiwen Ren

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Laboratory investigation; a journal of technical methods and pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 23 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. SCAInternational journal of molecular sciences · 2022
    Article
  9. Article
  10. Sirtuin Family and Diabetic Kidney Disease.Frontiers in endocrinology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Che BianDepartment of Endocrinology and Metabolism, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
Haibo ZhangAdvanced Institute for Medical Sciences, Dalian Medical University, Dalian, Liaoning, China.
Jing GaoDepartment of Gerontology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Yuxia WangDepartment of Endocrinology and Metabolism, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
Jia LiDepartment of Endocrinology and Metabolism, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
Dan GuoDepartment of Endocrinology and Metabolism, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
Wei WangDepartment of Endocrinology and Metabolism, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
Yuling SongDepartment of Endocrinology and Metabolism, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
Yang WengDepartment of Gastroenterology, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
Huiwen RenAdvanced Institute for Medical Sciences, Dalian Medical University, Dalian, Liaoning, China. rhwcmu@163.com.ORCID 0000-0002-6037-8561
Fourth Affiliated Hospital of China Medical University · CNDalian Medical University · CNXinHua Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to determine the mechanism by which SIRT6 regulates glucolipid metabolism disorders. We detected histological and molecular changes in Sprague-Dawley rats as well as in BRL 3A and INS-1 cell lines subjected to overnutrition and starvation. SIRT6, SREBP1c, and glucolipid metabolism biomarkers were identified by fluorescence co-localization, real-time PCR, and western blotting. Gene silencing studies were performed. Recombinant SIRT6, AMPK agonist (AICAR), mTOR inhibitor (rapamycin), and liver X receptor (LXR) agonist (T0901317) were used to pre-treated in BRL 3A and INS-1 cells. Real-time PCR and western blotting were used to detect related proteins, and cell counting was utilized to detect proliferation. We obtained conflicting results; SIRT6 and SREBP1c appeared in both the liver and pancreas of high-fat and hungry rats. Recombinant SIRT6 alleviated the decrease in AMPKα and increase in mTORC1 (complex of mTOR, Raptor, and Rheb) caused by overnutrition. SIRT6 siRNA reversed the glucolipid metabolic disorders caused by the AMPK agonist and mTOR inhibitor but not by the LXR agonist. Taken together, our results demonstrate that SIRT6 regulates glycolipid metabolism through AMPKα-mTORC1 regulating SREBP1c in the liver and pancreas induced by overnutrition and starvation, independent of LXR.

Indexed as

Lipid MetabolismLiverPancreasSirtuinsSterol Regulatory Element Binding Protein 1AMP-Activated Protein KinasesAnimalsMechanistic Target of Rapamycin Complex 1OvernutritionRatsRats, Sprague-DawleySignal TransductionStarvationTOR Serine-Threonine KinasesAMP-Activated Protein KinasesMechanistic Target of Rapamycin Complex 1sirtuin 6, ratSirtuinsSrebf1 protein, ratSterol Regulatory Element Binding Protein 1TOR Serine-Threonine Kinases

Identifiers

PMID34923569
OpenAlexW4200342645

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.