Evidence map›Paper›PMID 34922585›Full record

ArticleJournal of translational medicine2021

Extracellular vesicles carrying miRNA-181b-5p affects the malignant progression of acute lymphoblastic leukemia.

Wei Yan, Li Song, Huihan Wang, Wei Yang, Liang Hu, Ying Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  17. MiRNAs in Hematopoiesis and Acute Lymphoblastic Leukemia.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Wei YanDepartment of Hematology, Shengjing Hospital of China Medical University, Shenyang, 110000, People's Republic of China.
Li SongNational Drug Clinical Trial Institute Office, Qingdao Women and Children's Hospital, Qingdao, China.
Huihan WangDepartment of Hematology, Shengjing Hospital of China Medical University, Shenyang, 110000, People's Republic of China.
Wei YangDepartment of Hematology, Shengjing Hospital of China Medical University, Shenyang, 110000, People's Republic of China.
Liang HuShanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China.
Ying YangDepartment of Hematology, Shengjing Hospital of China Medical University, Shenyang, 110000, People's Republic of China. sj_yangy@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate how serum extracellular vesicles (EVs)-carried miRNA-181b-5p affected the proliferation, cell cycle and apoptosis of acute lymphoblastic leukemia (ALL) cells.

methodsDifferentially expressed miRNAs related to ALL were screened by bioinformatics analysis, and the localization of target miRNA was searched by its expression. qRT-PCR was adopted to confirm the expression of miRNA-181b-5p. Flow cytometry and fluorescence microscopy were applied to evaluate EVs internalization. MTT assay was employed to verify the proliferation of ALL cells. Cell cycle and apoptosis were analyzed by flow cytometry. Transwell assay was applied to evaluate migration and invasion abilities.

resultsHigh expression of miRNA-181b-5p was proved in ALL cell lines, and miRNA-181b-5p enriched in the exosomes and vesicles of blood cells. In the meantime, it was found that EVs carrying miRNA-181b-5p could be internalized by ALL cells and thus the expression of miRNA-181b-5p was up-regulated. Cell function assays showed that the proliferation, migration, invasion abilities of ALL cell lines were promoted in miRNA-181b-5p mimic group or the group co-culturing ALL-derived EVs and BALL-1 cell lines. The percentage of cells in G0/G1 phase was reduced and cell apoptosis was also inhibited.

conclusionmiRNA-181b-5p carried by EVs in peripheral blood of ALL patients can enter ALL cells and thus promote the malignancy of ALL cells.

Indexed as

ExosomesExtracellular VesiclesMicroRNAsPrecursor Cell Lymphoblastic Leukemia-LymphomaApoptosisHumansMicroRNAsALLCell cycleExtracellular vesiclesmiRNA-181b-5pProliferation

Identifiers

PMID34922585
PMCPMC8684212

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.