Evidence map›Paper›PMID 34921841›Full record

ArticleJournal of virological methods2022

COVID-19 PCR test performance on samples stored at ambient temperature.

Nihat Bugra Agaoglu, Jale Yildiz, Ozlem Akgun Dogan, Betsi Kose, Gizem Alkurt, Yasemin Kendir Demirkol, Arzu Irvem, Levent Doganay, Gizem Dinler Doganay

Open access · greenAbstract read
In one paragraph

Article in Journal of virological methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Nihat Bugra AgaogluGenomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey; Department of Medical Genetics, Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey.
Jale YildizGenomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey; Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul, Turkey.
Ozlem Akgun DoganGenomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey; Department of Pediatric Genetics, Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey.
Betsi KoseGenomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey.
Gizem AlkurtGenomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey; Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul, Turkey.
Yasemin Kendir DemirkolGenomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey; Department of Medical Genetics, Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey.
Arzu IrvemDepartment of Microbiology, Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey.
Levent DoganayGenomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey; Department of Gastroenterology, Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey.
Gizem Dinler DoganayGenomic Laboratory (GLAB), Umraniye Teaching and Research Hospital, University of Health Sciences, Istanbul, Turkey; Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul, Turkey. Electronic address: gddoganay@itu.edu.tr.
Sağlık Bilimleri Üniversitesi · TRÜmraniye Eğitim ve Araştırma Hastanesi · TRIstanbul Technical University · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The WHO-named Coronavirus Disease 2019 (COVID-19) infection had become a pandemic within a short time period since it was detected in Wuhan. The outbreak required the screening of millions of samples daily and overwhelmed diagnostic laboratories worldwide. During this pandemic, the handling of patient specimens according to the universal guidelines was extremely difficult as the WHO, CDC and ECDC required cold chain compliance during transport and storage of the swab samples. The aim of this study was to compare the effects of two different storage conditions on the COVID-19 real-time PCR assay on 30 positive nasopharyngeal and/or oropharyngeal samples stored at both ambient temperature (22 ± 2 °C) and +4 °C. The results revealed that all the samples stored at ambient temperature remain PCR positive for at least six days without any false-negative result. In conclusion, transporting and storing these types of swab samples at ambient temperature for six days under resource-limited conditions during the COVID-19 pandemics are acceptable.

Indexed as

COVID-19HumansPandemicsReal-Time Polymerase Chain ReactionSARS-CoV-2Specimen HandlingTemperatureCOVID-19Real-time PCRSample storage

Identifiers

PMID34921841
PMCPMC8673954
OpenAlexW4200523415

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.