Evaluation of the detection of staA, viaB and sopE genes in Salmonella spp. using the polymerase chain reaction (PCR).

Francis Kariuki, Pauline Getanda, Atunga Nyachieo, Gerald Juma, Peter Kinyanjui, Joseph Kamau

Abstract read

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Article in Archives of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

Establishment of methods for the detection ofVeterinary and animal science · 2026
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Authors and funding

6 authors.

Francis Kariuki *Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya. franciskariuki@gmail.com.ORCID http://orcid.org/0000-0002-4133-4109

Pauline Getanda *Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.

Atunga Nyachieo *Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.ORCID http://orcid.org/0000-0002-7906-5311

Gerald Juma *Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.

Peter Kinyanjui *Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.

Joseph Kamau *Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Typhoid fever is caused by the bacteria Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) and remains a significant health problem in many developing countries. Lack of adequate diagnostic capabilities has contributed greatly in making typhoid fever endemic in these regions. Reliable and inexpensive diagnostic tests are needed to improve the management of this disease burden. We evaluated the ability of staA, viaB and sopE genes to detect and differentiate between the three most prevalent Salmonella spp. in Kenya (S. Typhi, S. Typhimurium and S. Enteritidis) using conventional polymerase chain reaction (PCR). The staA primers and viaB primers were found to be specific only for the different strains of S. Typhi, producing PCR products of 585 bp and 540 bp, respectively. The sopE primers was demonstrated to be specific for all Salmonella spp. producing a 465 bp PCR product with no amplification with E. coli and S. boydii bacterial strains.

Indexed as

Escherichia coliSalmonellaKenyaPolymerase Chain ReactionSerogroupConventional PCRDiagnosisSalmonella TyphiTyphoid fever

Identifiers

PMID34921325

DOI10.1186/1476-0711-9-14

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.