ArticleScientific reports2021
Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 33 citations in OpenAlex.
- A versatile fluorescent reporter for Usutu virus drug screening and infection tracking.PNAS nexus · 2026Article
- First Detection of Usutu Virus in Harbor Seals (Viruses · 2026Article
- Neuropathogenesis of Usutu virus-associated disease in Eurasian blackbirds (Turdus merula) involves apoptosis through the extrinsic pathway.Journal of neurovirology · 2026Article
- Divergent roles of Hsp70 chaperones in orthoflavivirus protein secretion and virion formation.Npj viruses · 2026Article
- One Health Approach in the Era of Emerging Viruses: The Challenge of Usutu Virus.International journal of molecular sciences · 2025Review
- STAT1-mediated interferon signaling in the hematopoietic system is essential for restricting Usutu virus infection in vivo.PLoS neglected tropical diseases · 2025Article
- Article
- Herpes simplex virus assembly and spread in murine skin after infection from the outside.Journal of virology · 2025Article
- Usutu virus continues to spread across Europe: first report of multiple molecular detections of the USUV Africa 2 and Africa 3 lineages in free-living and captive birds in Poland, July-November 2023.Veterinary research · 2025Article
- Mosquito- and tick-borne orthoflaviviruses cross anFrontiers in cellular and infection microbiology · 2025Article
- Evaluating the potential of anti-dsRNA antibodies as an alternative viral sensing tool in encephalitides of different species.Frontiers in veterinary science · 2025Article
- Double-Stranded RNA-Based Method for Diagnosing Severe Fever with Thrombocytopenia.Journal of clinical medicine · 2024Article
- Emerging mosquito-borne flaviviruses.mBio · 2024Review
- Is Culex modestus a New Usutu virus vector?Parasites & vectors · 2024Article
- Reconstruction of the molecular evolution of Usutu virus in Germany: Insights into virus emersion and circulation.PLoS neglected tropical diseases · 2023Article
- Epidemiology, surveillance and diagnosis of Usutu virus infection in the EU/EEA, 2012 to 2021.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2023Review
- A Prior Usutu Virus Infection Can Protect Geese from Severe West Nile Disease.Pathogens (Basel, Switzerland) · 2023Article
- Pathological features of West Nile and Usutu virus natural infections in wild and domestic animals and in humans: A comparative review.One health (Amsterdam, Netherlands) · 2023Article
- Longitudinal Study of the Occurrence of Usutu Virus and West Nile Virus Infections in Birds in a Zoological Garden in Northern Germany.Pathogens (Basel, Switzerland) · 2023Article
- Rustrela Virus as Putative Cause of Nonsuppurative Meningoencephalitis in Lions.Emerging infectious diseases · 2023Article
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Usutu virus (USUV) is a zoonotic arbovirus causing avian mass mortalities. The first outbreak in North-Western Germany occurred in 2018. This retrospective analysis focused on combining virological and pathological findings in birds and immunohistochemistry. 25 common blackbirds, one great grey owl, and one kingfisher collected from 2011 to 2018 and positive for USUV by qRT-PCR were investigated. Macroscopically, most USUV infected birds showed splenomegaly and hepatomegaly. Histopathological lesions included necrosis and lymphohistiocytic inflammation within spleen, Bursa fabricii, liver, heart, brain, lung and intestine. Immunohistochemistry revealed USUV antigen positive cells in heart, spleen, pancreas, lung, brain, proventriculus/gizzard, Bursa fabricii, kidney, intestine, skeletal muscle, and liver. Analysis of viral genome allocated the virus to Europe 3 or Africa 2 lineage. This study investigated whether immunohistochemical detection of double-stranded ribonucleic acid (dsRNA) serves as an alternative tool to detect viral intermediates. Tissue samples of six animals with confirmed USUV infection by qRT-PCR but lacking viral antigen in liver and spleen, were further examined immunohistochemically. Two animals exhibited a positive signal for dsRNA. This could indicate either an early state of infection without sufficient formation of virus translation products, occurrence of another concurrent virus infection or endogenous dsRNA not related to infectious pathogens and should be investigated in more detail in future studies.
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