Evidence map›Paper›PMID 34920936›Full record

ReviewTrends in cell biology2022

Intravital and high-content multiplex imaging of the immune system.

Jyh Liang Hor, Ronald N Germain

Abstract readReview
In one paragraph

Review in Trends in cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Article
  3. A modeller's guide for biomedical discovery.Disease models & mechanisms · 2026
    Article
  4. Review
  5. Article
  6. Article
  7. Correlative Imaging Platform Linking Taste Cell Function to Molecular Identity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Review
  15. Review
  16. Review
  17. Article
  18. Multiplex imaging in immuno-oncology.Journal for immunotherapy of cancer · 2023
    Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Jyh Liang HorLymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1892, USA.
Ronald N GermainLymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1892, USA. Electronic address: rgermain@niaid.nih.gov.

Funding

Multiscale Analysis of Immune ResponsesZIAAI000545 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI GERMAIN, RONALD N · 2009 to 2025
$29.4M
Multiscale Analysis of Immune ResponsesZ01AI000545 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI GERMAIN, RONALD N · 1990 to 2008
$4.5M
FUNCTIONAL BIOLOGY OF T CELLSZ01AI000758 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI GERMAIN, RONALD N · 1997 to 2008
$1.2M
Intramural NIH HHS Z01 AI000545Intramural NIH HHS Z01 AI000758
6 · The paper itself

Abstract

Highly motile and functionally diverse immune cells orchestrate effective immune responses through complex and dynamic cooperative behavior. Multiphoton intravital microscopy (MP-IVM) presents a unique and powerful tool to study the coordinated action of immune cell interactions in situ. Here, we review the current state of intravital microscopy in deepening our understanding of the immune system and discuss its fundamental limitations. In addition, we draw insights from recent technical advances in multiplex static tissue-imaging methods and propose an approach that could enable simultaneous visualization of cellular dynamics, deep phenotyping, and transcriptional states through a new type of correlative microscopy that combines these imaging technologies with advances in complex data analysis.

Indexed as

Intravital MicroscopyMicroscopy, Fluorescence, MultiphotonCell CommunicationHumansImmune Systemcorrelative microscopyfluorescence microscopyintravital imagingiterative stainingmulti-photon

Identifiers

PMID34920936
PMCPMC9018524

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.