ArticleEuropean journal of medical research2021
Establishment of a quantitative RT-PCR detection of SARS-CoV-2 virus.
Article in European journal of medical research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Trial
- Age, sex and seasonal differences in COVID-19 viral load: a Lebanese cross-sectional study.BMC infectious diseases · 2025Article
- Traditional medicine meets modern science: Halofuginone's role in combating autoimmune diseases.Journal of natural medicines · 2025Review
- Vaccination in twin pregnancies: comparison between immunization before conception and during pregnancy.Scientific reports · 2024Article
- Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection ofMicroorganisms · 2023Article
- Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection.Sensors (Basel, Switzerland) · 2023Article
- SARS-CoV-2 viral RNA detection using the novel CoVradar device associated with the CoVreader smartphone app.Biosensors & bioelectronics · 2023Article
- Influence of SARS-COV-2 Infection on Cytokine Production by Mitogen-Stimulated Peripheral Blood Mononuclear Cells and Neutrophils in COVID-19 Intensive Care Unit Patients.Microorganisms · 2022Article
- Construction of ratiometric Si-Mn:ZnSe nanoparticles for the immunoassay of SARS-CoV-2 spike protein.Sensors and actuators. B, Chemical · 2022Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe outbreak of novel coronavirus disease 2019 (COVID-19) has become a public health emergency of international concern. Quantitative testing of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus is demanded in evaluating the efficacy of antiviral drugs and vaccines and RT-PCR can be widely deployed in the clinical assay of viral loads. Here, we developed a quantitative RT-PCR method for SARS-CoV-2 virus detection in this study.
methodsRT-PCR kits targeting E (envelope) gene, N (nucleocapsid) gene and RdRP (RNA-dependent RNA polymerase) gene of SARS-CoV-2 from Roche Diagnostics were evaluated and E gene kit was employed for quantitative detection of COVID-19 virus using Cobas Z480. Viral load was calculated according to the standard curve established by series dilution of an E-gene RNA standard provided by Tib-Molbiol (a division of Roche Diagnostics). Assay performance was evaluated.
resultsThe performance of the assay is acceptable with limit of detection (LOD) below 10E1 copies/μL and lower limit of quantification (LLOQ) as 10E2 copies/μL.
conclusionA quantitative detection of the COVID-19 virus based on RT-PCR was established.
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Registered trials
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